Gene Expression Data
Assay Details
Assay
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Reference:
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J:265753
Bao J, et al., The arginine methyltransferase CARM1 represses p300*ACT*CREMtau activity and is required for spermiogenesis. Nucleic Acids Res. 2018 May 18;46(9):4327-4343
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6283454
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Gene symbol:
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Carm1
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Gene name:
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coactivator-associated arginine methyltransferase 1
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Antibody:
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anti-Carm1
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Assay notes:
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The expression intensity and localization were summarized in (E).
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Results
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Specimen
1D Stage V:
postnatal adult
(more )
Specimen
1D Stage V:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS28: spermatocyte |
Present |
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1D upper
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Expressed in the cytoplasm.
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TS28: spermatid |
Present |
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1D upper
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Expression translocated to the nucleus, but was excluded from the heterochromatin structure referred to as Chromocenter.
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Specimen
1D Stage VIII:
postnatal adult
(more )
Specimen
1D Stage VIII:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: spermatid |
Present |
|
1D middle
|
Expression translocated to the nucleus, but was excluded from the heterochromatin structure referred to as Chromocenter.
|
Specimen
1D Stage X:
postnatal adult
(more )
Specimen
1D Stage X:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Male
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: spermatid |
Present |
|
1D lower
|
Highest expression in the nuclei of elongating spermatids.
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Specimen
not shown:
postnatal adult
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
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