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Gene Expression Data
Assay Details
Assay
Reference: J:183953 Francois M, et al., Segmental territories along the cardinal veins generate lymph sacs via a ballooning mechanism during embryonic lymphangiogenesis in mice. Dev Biol. 2012 Apr 15;364(2):89-98
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6283364
Gene symbol: Prox1
Gene name: prospero homeobox 1
Antibody: anti-Prox1 (#11-002)
Detection system: Alexa Fluor 555
Assay notes: Two antibodies were described, but the one used in this assay was not detailed. Prox1 antibody MGI:5486156 may have been used in this assay.
Results
Specimen 2D: embryonic day 11.5 (more )
Note: Triple labeled: red - Prox1; green - Pdpn (assay MGI:6283374); blue - Cd34 (assay MGI:6283375).
Structure Level Pattern Image Note
TS19: lymphatic system Present Regionally restricted 2D Expression in lymphatic endothelial cell precursors delaminated from the cardinal vein.
Specimen 2E: embryonic day 11.5 (more )
Note: Triple labeled: red - Prox1; green - Pdpn (assay MGI:6283374); blue - Cd34 (assay MGI:6283375).
Structure Level Pattern Image Note
TS19: lymphatic system Present Regionally restricted 2E Expression in pre-lymphatic clusters (PLC) at level of forelimb bud where cardinal vein diverts to form a lateral branch.
Specimen 2F: embryonic day 11.5 (more )
Note: Triple labeled: red - Prox1; green - Pdpn (assay MGI:6283374); blue - Cd34 (assay MGI:6283375).
Structure Level Pattern Image Note
TS19: lymphatic system Present Regionally restricted 2F Expression in pre-lymphatic clusters (PLC) at level of forelimb bud where cardinal vein diverts to form a lateral branch.
Specimen 2G: embryonic day 11.5 (more )
Note: Double labeled: red - Prox1; green - Ter119 (Ly76 assay MGI:6283376).
Structure Level Pattern Image Note
TS19: lymph sac Present 2G
Specimen 2H: embryonic day 11.5 (more )
Note: Double labeled: red - Prox1; green - Ter119 (Ly76 assay MGI:6283376).
Structure Level Pattern Image Note
TS19: lymph sac Present 2H
Specimen 2I: embryonic day 11.5 (more )
Note: Double labeled: red - Prox1; green - Ter119 (Ly76 assay MGI:6283376).
Structure Level Pattern Image Note
TS19: lymph sac Present 2I
Specimen 3D: embryonic day 11.5 (more )
Note: Triple labeled: red - Prox1; green - Pdpn (assay MGI:6283374); blue - Pecam1 (assay MGI:6283367).
Structure Level Pattern Image Note
TS19: lymph sac Present 3D
TS19: lymphatic system Present Regionally restricted 3D Authors report lymph sacs were observed immediately adjacent to pre-lymphatic clusters.
Specimen 3E: embryonic day 11.5 (more )
Note: Triple labeled: red - Prox1; green - Pdpn (assay MGI:6283374); blue - Pecam1 (assay MGI:6283367).
Structure Level Pattern Image Note
TS19: lymph sac Present 3E
TS19: lymphatic system Present Regionally restricted 3E Authors report lymph sacs were observed immediately adjacent to pre-lymphatic clusters.
Specimen 3F: embryonic day 11.5 (more )
Note: Triple labeled: red - Prox1; green - Pdpn (assay MGI:6283374); blue - Pecam1 (assay MGI:6283367).
Structure Level Pattern Image Note
TS19: lymph sac Present 3F
TS19: lymphatic system Present Regionally restricted 3F Authors report lymph sacs were observed immediately adjacent to pre-lymphatic clusters.
Specimen 4B: embryonic day 11.5 (more )
Note: Double labeled: red - Prox1; green - Pecam1 (assay MGI:6283370).
Structure Level Pattern Image Note
TS19: lymph sac Present Regionally restricted 4B Expression in lymph sac endothelium.
Specimen 4C: embryonic day 11.5 (more )
Note: Double labeled: red - Prox1; green - Pecam1 (assay MGI:6283370).
Structure Level Pattern Image Note
TS19: lymph sac Present Regionally restricted 4C Expression in lymph sac endothelium.
Specimen 4D: embryonic day 11.5 (more )
Note: Double labeled: red - Prox1; green - Pecam1 (assay MGI:6283370).
Structure Level Pattern Image Note
TS19: lymph sac Present Regionally restricted 4D Expression in lymph sac endothelium.
Specimen 4E: embryonic day 11.5 (more )
Note: Double labeled: red - Prox1; green - Pecam1 (assay MGI:6283370).
Structure Level Pattern Image Note
TS19: lymph sac Present Regionally restricted 4E Expression in lymph sac endothelium.
Specimen 4F: embryonic day 11.5 (more )
Note: Double labeled: red - Prox1; green - Pecam1 (assay MGI:6283370).
Structure Level Pattern Image Note
TS19: lymph sac Present Regionally restricted 4F Expression in lymph sac endothelium.
Specimen 4G: embryonic day 11.5 (more )
Note: Double labeled: red - Prox1; green - Pecam1 (assay MGI:6283370).
Structure Level Pattern Image Note
TS19: lymph sac Present Regionally restricted 4G Expression in lymph sac endothelium.
Specimen 4H: embryonic day 11.5 (more )
Note: Double labeled: red - Prox1; green - Pecam1 (assay MGI:6283370).
Structure Level Pattern Image Note
TS19: lymph sac Present Regionally restricted 4H Expression in lymph sac endothelium.
Specimen 4I: embryonic day 11.5 (more )
Note: Double labeled: red - Prox1; green - Pecam1 (assay MGI:6283370).
Structure Level Pattern Image Note
TS19: lymph sac Present Regionally restricted 4I Expression in lymph sac endothelium.
Specimen 4J: embryonic day 11.5 (more )
Note: Double labeled: red - Prox1; green - Pecam1 (assay MGI:6283370).
Structure Level Pattern Image Note
TS19: lymph sac Present Regionally restricted 4J Expression in lymph sac endothelium.
Specimen 4K: embryonic day 11.5 (more )
Note: Double labeled: red - Prox1; green - Pecam1 (assay MGI:6283370).
Structure Level Pattern Image Note
TS19: lymph sac Present Regionally restricted 4K Expression in lymph sac endothelium.
Specimen 4L: embryonic day 11.5 (more )
Note: Double labeled: red - Prox1; green - Pecam1 (assay MGI:6283370).
Structure Level Pattern Image Note
TS19: lymph sac Present Regionally restricted 4L Expression in lymph sac endothelium.
Specimen 5A/C: embryonic day 11.5 (more )
Note: Triple labeled: red - Prox1; green - Emcn (assay MGI:6283377); cyan - Lyve1 (assay MGI:6283372).
Structure Level Pattern Image Note
TS19: lymph sac Present Regionally restricted 5A , 5C
Specimen 5E/G: embryonic day 10.5 (more )
Note: Triple labeled: red - Prox1; green - Emcn (assay MGI:6283377); cyan - Nrp2 (assay MGI:6283378).
Structure Level Pattern Image Note
TS17: lymphatic system Present Regionally restricted 5E , 5G Expression in lymph sacs maintaining a stalklike connection to the cardinal vein at these stages of development. Expression in lymphatic endothelial cells sprouting in a dorso-lateral direction towards the embryo periphery.
Specimen 5I/K: embryonic day 10.0 (more )
Note: Triple labeled: red - Prox1; green - Emcn (assay MGI:6283377); cyan - Lyve1 (assay MGI:6283372).
Structure Level Pattern Image Note
TS16: cardinal vein Present Regionally restricted 5K , 5I Expression in cells migrating directly from cardinal vein prior to lymph sac formation.
Specimen 5H/O: embryonic day 10.0 (more )
Note: Triple labeled: red - Prox1; green - Emcn (assay MGI:6283377); cyan - Nrp2 (assay MGI:6283378).
Structure Level Pattern Image Note
TS16: cardinal vein Present Regionally restricted 5M , 5O Expression in cells migrating directly from cardinal vein prior to lymph sac formation.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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