Gene Expression Data
Assay Details
Assay
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Reference:
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J:182713
McDonel P, et al., Sin3a is essential for the genome integrity and viability of pluripotent cells. Dev Biol. 2012 Mar 1;363(1):62-73
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6283040
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Gene symbol:
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Nanog
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Gene name:
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Nanog homeobox
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Antibody:
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anti-Nanog (ab21603)
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Assay notes:
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Fixation with 2.5% paraformaldehyde. Two antibodies were described, but the one used in this assay was not detailed. Nanog antibody MGI:6241547 may have been used in this assay.
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Results
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Specimen
1C Nanog +/+:
embryonic day 3.5
(more )
Specimen
1C Nanog +/+:
(close )
Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Specimen
1C Nanog -/-:
embryonic day 3.5; Sin3atm1.1Rdp/Sin3a +
(more )
Specimen
1C Nanog -/-:
(close )
Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Sin3atm1.1Rdp/Sin3a+
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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