Sin3a Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:182713 McDonel P, et al., Sin3a is essential for the genome integrity and viability of pluripotent cells. Dev Biol. 2012 Mar 1;363(1):62-73
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6283036
Gene symbol:	Sin3a
Gene name:	transcriptional regulator, SIN3A (yeast)

Antibody:	anti-Sin3B (AD-12)
Assay notes:	Fixation with 2.5% paraformaldehyde.

Results

Specimen 1A Sin3A 2-cell +/+: embryonic day 1.0 (more

)

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Present	Regionally restricted	1A 2-cell Sin3A +/+	Authors report maternal expression in nuclei of wild type and homozygous mutant embryos.

Specimen 1A Sin3A 2-cell -/-: embryonic day 1.0; Sin3a^tm1.1Rdp/Sin3a^tm1.1Rdp (more

)

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Present	Regionally restricted	1A 2-cell Sin3A -/-	Authors report maternal expression in nuclei of wild type and homozygous mutant embryos.

Specimen 1B Sin3A 8-cell +/+: embryonic day 2.5 (more

)

Structure	Level	Pattern	Image	Note
TS3: 8-cell stage embryo	Present	Regionally restricted	1B 8-cell Sin3A +/+	Nuclear expression in wild type and heterozygous mutant embryos.

Specimen Sin3A 8-cell +/- not shown: embryonic day 2.5; Sin3a^tm1.1Rdp/Sin3a⁺ (more

)

Structure	Level	Pattern	Image	Note
TS3: 8-cell stage embryo	Present	Regionally restricted		Nuclear expression in wild type and heterozygous mutant embryos.

Specimen 1B Sin3A 8-cell -/-: embryonic day 2.5; Sin3a^tm1.1Rdp/Sin3a^tm1.1Rdp (more

)

Structure	Level	Pattern	Image	Note
TS3: 8-cell stage embryo	Weak	Regionally restricted	1B 8-cell Sin3A -/-	Nuclear expression is less apparent than in wild type littermates.

Specimen 1B Sin3A 16-cell +/+: embryonic day 3.0 (more

)

Structure	Level	Pattern	Image	Note
TS3: compacted morula	Present	Regionally restricted	1B 16-cell Sin3A +/+	Nuclear expression in wild type and heterozygous mutant embryos.

Specimen Sin3A 16-cell +/- not shown: embryonic day 3.0; Sin3a^tm1.1Rdp/Sin3a⁺ (more

)

Structure	Level	Pattern	Image	Note
TS3: compacted morula	Present	Regionally restricted		Nuclear expression in wild type and heterozygous mutant embryos.

Specimen 1B Sin3A 16-cell -/-: embryonic day 3.0; Sin3a^tm1.1Rdp/Sin3a^tm1.1Rdp (more

)

Structure	Level	Pattern	Image	Note
TS3: compacted morula	Ambiguous		1B 16-cell Sin3A -/-	Authors state maternal protein has largely been depleted. No nuclear expression.

Specimen 1B Sin3A Blastocyst +/+: embryonic day 3.5 (more

)

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Present	Ubiquitous	1B Blastocyst Sin3A +/+	Ubiquitous expression in wild type and heterozygous mutant embryos.

Specimen Sin3A Blastocyst +/- not shown: embryonic day 3.5; Sin3a^tm1.1Rdp/Sin3a⁺ (more

)

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Present	Ubiquitous		Ubiquitous expression in wild type and heterozygous mutant embryos.

Specimen 1B Sin3A Blastocyst -/-: embryonic day 3.5; Sin3a^tm1.1Rdp/Sin3a^tm1.1Rdp (more

)

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Ambiguous	Regionally restricted	1B Blastocyst Sin3A -/-

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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