Gene Expression Data
Assay Details
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Assay
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Reference:
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J:32851
Andermarcher E, et al., Co-expression of the HGF/SF and c-met genes during early mouse embryogenesis precedes reciprocal expression in adjacent tissues during organogenesis. Dev Genet. 1996;18(3):254-66
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6281714
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Gene symbol:
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Met
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Gene name:
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met proto-oncogene, receptor tyrosine kinase
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Probe:
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c-met cDNA1 (clone B.2)
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
pre-streak stage not shown:
embryonic day 6.25
(more  )
Specimen
pre-streak stage not shown:
(close  )
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Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 6.25
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS8: embryo |
Absent |
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No expression before onset of gastrulation.
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Specimen
1B:
embryonic day 6.5-6.75
(more )
Note: early-mid-streak stage
Specimen
1B:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 6.5-6.75
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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early-mid-streak stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS9: primitive streak |
Strong |
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1B
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At onset of gastrulation, strong expression in region of the developing primitive streak.
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Specimen
1E:
embryonic day 6.75-7.0
(more )
Note: mid-late streak stage
Specimen
1E:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 6.75-7.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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mid-late streak stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS10: primitive streak |
Present |
Regionally restricted |
1E
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Expression follows the spatial progreassion of the streak and becomes more localized in intermediate and rostral part of the streak.
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| TS10: node |
Present |
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1E
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Starting at the late primitive streak, with the first appearance of the node, expression in region corresponding to the node.
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Specimen
mid-late streak stage not shown:
embryonic day 6.75-7.0
(more )
Specimen
mid-late streak stage not shown:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 6.75-7.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS10: embryo mesoderm |
Present |
Regionally restricted |
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Expression in the area of heart mesoderm from which the primordium of the heart will differentiate.
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| TS10: definitive endoderm |
Present |
Regionally restricted |
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Expression in foregut endoderm.
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Specimen
neural plate / headfold stage not shown:
embryonic day 7.5
(more )
Specimen
neural plate / headfold stage not shown:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: node |
Absent |
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Specimen
1H:
embryonic day 7.75
(more )
Note: late headfold stage
Specimen
1H:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 7.75
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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late headfold stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: node |
Present |
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1H
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| TS11: neural groove |
Present |
Regionally restricted |
1H
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Expression localized in intermediate and rostral part of neural groove, corresponding to area where notochord is forming.
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| TS11: definitive endoderm |
Present |
Regionally restricted |
1H
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Expression in the prospective foregut.
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| TS11: future brain |
Present |
Regionally restricted |
1H
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Transient expression in prospective forebrain.
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Specimen
4-5 somite stage not shown:
embryonic day 8.0
(more )
Specimen
4-5 somite stage not shown:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: common atrial chamber |
Strong |
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High levels of expression in common atrium and ventricle. Expression overlaps with HGF/SF in both myocardial and endocardial tissues.
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| TS12: primitive ventricle |
Strong |
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High levels of expression in common atrium and ventricle. Expression overlaps with HGF/SF in both myocardial and endocardial tissues.
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| TS12: somite |
Present |
Uniform |
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Expression evenly distributed over newly formed somites, overlapping with that of HGF/SF
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Specimen
1K:
embryonic day 10.0
(more )
Note: 30-35 somite stage
Specimen
1K:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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30-35 somite stage
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Specimen
2B:
embryonic day 6.75-7.0
(more )
Specimen
2B:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 6.75-7.0
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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