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Tg(Acta2-RHO/Adrb2,-lacZ)B7-5Mik
Transgene Detail
Summary
Symbol: Tg(Acta2-RHO/Adrb2,-lacZ)B7-5Mik
Name: transgene insertion B7-5, Michael I Kotlikoff
MGI ID: MGI:6281620
Synonyms: Acta2-Opto beta;2AR-IRES-lacZ, B7-Acat2BAC-Opto beta 2AR_IRES_lacZ
Transgene: Tg(Acta2-RHO/Adrb2,-lacZ)B7-5Mik  Location: unknown  
Alliance: Tg(Acta2-RHO/Adrb2,-lacZ)B7-5Mik page
Transgene
origin
Strain of Origin:  FVB/N X B6(Cg)-Tyrc-2J/J
Transgene
description
Transgene Type:    Transgenic (Inserted expressed sequence, Reporter)
Mutation:    Insertion
 
Mutation detailsThe transgenic construct is made using a C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-370F21 containing the entire actin alpha 2 locus (Acta2) as well as ~92 kbp of 5' flanking sequences (including the complete Fas locus) and ~68 kbp of 3' flanking sequences (including the complete Stambpl1 locus). Using homologous recombination/BAC recombineering, a 5990 bp Opto alpha;1AR-IRES-lacZ-pA construct is inserted into the ATG start site of the BAC Acta2 gene (replacing the initiation codon in exon 2). No other loci on the BAC are altered. The 11.6 kbp BAC vector (pBACe3.6) contains a chloramphenicol selection cassette. The Opto beta 2AR fusion protein is a Gt-coupled bovine rhodopsin (RHO) sequence modified to have its intracellular regions (including carboxy-terminal domains) replaced with those from the Gs-coupled hamster Adrb2. Founder line B7-5 has high sensor expression in smooth muscle. (J:314125)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:314125 Lee FK, et al., Genetically engineered mice for combinatorial cardiovascular optobiology. Elife. 2021 Oct 29;10:e67858
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/19/2024
MGI 6.23
The Jackson Laboratory