Tg(Camk2a*T286D)#Mmay
Transgene Detail
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| Symbol: |
Tg(Camk2a*T286D)#Mmay |
| Name: |
transgene insertion, Mark Mayford |
| MGI ID: |
MGI:6281412 |
| Synonyms: |
CaMKII-Asp-286 |
| Transgene: |
Tg(Camk2a*T286D)#Mmay Location: unknown
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| Alliance: |
Tg(Camk2a*T286D)#Mmay page
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| Strain of Origin: |
Not Specified
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| Transgene Type: |
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Transgenic (Inserted expressed sequence) |
| Mutation: |
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Insertion
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Tg(Camk2a*T286D)#Mmay expresses
1 gene
Transgene expresses:
| Organism |
Expressed Gene |
Note |
| mouse |
Camk2a (MGI:88256) |
mouse promoter and gene (5' part genomic, 3' part mutagenized cDNA) |
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Mutation details: Genomic sequence starting 8.5 kb upstream of the translation initiation site and containing some 5' exons, was fused to a cDNA containing the remaining 3' sequence (including 3' UTR). The cDNA had been mutagenized to change threonine codon 286 (ACC) to an aspartic acid codon (GAC) (p.286D), which creates a phosphomimic in the peptide. The mutant peptide is 20- to 30-fold more active as a kinase in the absence of Ca(2+) than the wild-type form. Three mouse lines successfully transmitted the allele, and lines P3 and P15 were studied further. Northern blot and RT-PCR experiments showed expression of the transgene. The pound # sign in the symbol for this allele is used where no line is specified.
(J:271403)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Original: |
J:271403 Mayford M, et al., CaMKII regulates the frequency-response function of hippocampal synapses for the production of both LTD and LTP. Cell. 1995 Jun 16;81(6):891-904 |
| All: |
1 reference(s) |
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