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Gene Expression Data
Assay Details
Assay
Reference: J:170581 Kwon HJ, et al., Radial glia regulate Cajal-Retzius cell positioning in the early embryonic cerebral cortex. Dev Biol. 2011 Mar 1;351(1):25-34
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6281170
Gene symbol: Nes
Gene name: nestin
Antibody: clone RC2
Detection system: Secondary antibody coupled to Cy3
Results
Specimen 2A': embryonic day 15.5; Itgb1tm1Mll/? (more )
Note: Double labeled: red - Nes; green - PH3. Controls were either homozygous animals without cre, or heterozygotes with cre.
Structure Level Pattern Image Note
TS23: cerebral cortex subventricular zone Present 2A' , 2A'' Expression was detected in radial glia fibers that formed a scaffold spanning the ventricular and subventricular zones.
TS23: cerebral cortex ventricular layer Present 2A' , 2A'' Expression was detected in radial glia fibers that formed a scaffold spanning the ventricular and subventricular zones.
Specimen 2B': embryonic day 15.5; Itgb1tm1Mll/Itgb1tm1Mll, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: red - Nes; green - PH3.
Structure Level Pattern Image Note
TS23: cerebral cortex subventricular zone Present 2B'' , 2B' The staining pattern observed in the mutant was similar to wild type.
TS23: cerebral cortex ventricular layer Present 2B'' , 2B' The staining pattern observed in the mutant was similar to wild type.
Specimen 4A': embryonic day 15.5; Itgb1tm1Mll/? (more )
Note: Double labeled: red - Nes; green - Calb2 (assay MGI:6281165). Controls were either homozygous animals without cre, or heterozygotes with cre.
Structure Level Pattern Image Note
TS23: cerebral cortex Present 4A'' , 4A' Expression was detected in radial glia in a parallel scaffold spanning the cortical wall.
Specimen 4B': embryonic day 15.5; Itgb1tm1Mll/Itgb1tm1Mll, Wnt3atm1(cre)Eag/Wnt3a+ (more )
Note: Conditional mutant. Double labeled: red - Nes; green - Calb2 (assay MGI:6281165).
Structure Level Pattern Image Note
TS23: cerebral cortex Present 4B' , 4B'' In the mutant, expression was detected in radial glia organized as in control.
Specimen 5A': embryonic day 14.5; Itgb1tm1Mll/? (more )
Note: Double labeled: red - Nes; green - Calb2 (assay MGI:6281165). Controls were either homozygous animals without cre, or heterozygotes with cre.
Structure Level Pattern Image Note
TS22: cerebral cortex Present 5A'' , 5A' Expression was detected in radial glia fibers that formed a parallel scaffold.
Specimen 5B': embryonic day 14.5; Itgb1tm1Mll/Itgb1tm1Mll, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: red - Nes; green - Calb2 (assay MGI:6281165).
Structure Level Pattern Image Note
TS22: cerebral cortex Present 5B' , 5B'' Expression was detected in the radial glia fibers. Consistent disruptions in the radial glia scaffold were observed across the cortex of the mutant. The web of staining normally associated with radial glia endfeet was substantially reduced in many regions of the mutant cortex.
Specimen 7A: embryonic day 14.5; Orc3tm1.1Zhua/? (more )
Note: Double labeled: red - Nes; green - laminin. Controls were either homozygous animals without cre, or heterozygotes with cre.
Structure Level Pattern Image Note
TS22: cerebral cortex Present 7A'' , 7A Expression was detected in dense and evenly spaced radial glia fibers.
Specimen 7B: embryonic day 14.5; Orc3tm1.1Zhua/Orc3tm1.1Zhua, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: red - Nes; green - laminin.
Structure Level Pattern Image Note
TS22: cerebral cortex Present 7B , 7B'' Expression was detected in the radial glia fibers, but there was a severe disruption in the radial glia scaffold, with frequent large gaps between a reduced number of glial fibers.
Specimen 8A: embryonic day 14.5; Orc3tm1.1Zhua/? (more )
Note: Double labeled: red - Nes; green - Calb2 (assay MGI:6281165). Controls were either homozygous animals without cre, or heterozygotes with cre.
Structure Level Pattern Image Note
TS22: cerebral cortex Present 8A' , 8A Expression was detected in an evenly spaced scaffold of radial glia fibers.
Specimen 8B: embryonic day 14.5; Orc3tm1.1Zhua/Orc3tm1.1Zhua, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: red - Nes; green - Calb2 (assay MGI:6281165).
Structure Level Pattern Image Note
TS22: cerebral cortex Present 8b , 8B , 8a , 8B'' Expression was detected in radial glia fibers. In the mutant cortex, frequent gaps appear in the scaffold of fibers.
Specimen S1A: embryonic day 15.5; Itgb1tm1Mll/? (more )
Note: Double labeled: red - Nes; green - Calb2 (assay MGI:6281165). Controls were either homozygous animals without cre, or heterozygotes with cre.
Structure Level Pattern Image Note
TS23: cerebral cortex Present S1A , S1A'' , S1a Expression was detected in radial glial fibers spanning the entire cortical wall. The endfeet were an intensely stained layer at the pial surface.
Specimen S1B: embryonic day 15.5; Itgb1tm1Mll/Itgb1tm1Mll, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: red - Nes; green - Calb2 (assay MGI:6281165).
Structure Level Pattern Image Note
TS23: cerebral cortex Present S1B'' , S1b , S1B In the mutant cortex, widespread retraction of the radial glial endfeet into the deeper cortex was observed.
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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