Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	Nfiatm1c(EUCOMM)Hmgu
Name:	nuclear factor I/A; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
MGI ID:	MGI:6280648
Synonyms:	NFIAfl, Nfiatm2Rmg
Gene:	Nfia  Location: Chr4:97660971-98007111 bp, + strand  Genetic Position: Chr4, 45.52 cM, cytoband C4-C6
Alliance:	Nfiatm1c(EUCOMM)Hmgu page
IMPC:	Nfia gene page

Germline Transmission:	Earliest citation of germline transmission: J:271120
Parent Cell Line:	JM8A3.N1 (ES Cell)
Strain of Origin:	C57BL/6N-Atm1Brd
Project Collection:	EUCOMM

Allele Type:    Targeted (Conditional ready) Mutation:    Insertion   Mutation details: The L1L2_Bact_P cassette was inserted at position 97670780 of Chromosome 4 upstream of exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 2 at position 97672189. Exon 2 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking exon 2. (J:271120)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Nfia Mutation:	28 strains or lines available

Original:	J:271120 Scavuzzo MA, et al., Pancreatic Cell Fate Determination Relies on Notch Ligand Trafficking by NFIA. Cell Rep. 2018 Dec 26;25(13):3811-3827.e7
All:	8 reference(s)