Gene Expression Data
Assay Details
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Assay
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Reference:
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J:176607
Kameda Y, et al., Hes1 regulates the number and anterior-posterior patterning of mesencephalic dopaminergic neurons at the mid/hindbrain boundary (isthmus). Dev Biol. 2011 Oct 1;358(1):91-101
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6280487
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Gene symbol:
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Otx2
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Gene name:
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orthodenticle homeobox 2
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Antibody:
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anti-Otx2
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
+/+ not shown:
embryonic day 11.5
(more  )
Specimen
+/+ not shown:
(close  )
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Genetic Background:
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involves: CD-1
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forebrain |
Present |
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Expression restricted to forebrain and midbrain, regions rostral to the isthmic organizer.
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| TS19: midbrain |
Present |
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Expression restricted to forebrain and midbrain, regions rostral to the isthmic organizer.
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Specimen
-/- not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Hes1tm1Fgu/Hes1tm1Fgu
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forebrain |
Present |
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Expression patterns similar to that of wild type embryos.
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| TS19: midbrain |
Present |
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Expression patterns similar to that of wild type embryos.
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Specimen
10A:
embryonic day 12.5
(more )
Specimen
10A:
(close )
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Genetic Background:
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involves: CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
10B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Hes1tm1Fgu/Hes1tm1Fgu
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: forebrain |
Present |
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10B
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Expression patterns similar to that of wild type embryos.
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| TS20: hindbrain |
Present |
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10B
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Expression patterns similar to that of wild type embryos.
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| TS20: midbrain |
Present |
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10B
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Expression patterns similar to that of wild type embryos.
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Specimen
10G:
embryonic day 14.5
(more )
Specimen
10G:
(close )
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Genetic Background:
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involves: CD-1
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
Present |
Regionally restricted |
10G
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Expression concentrated in mesencephalic dopaminergic (mesDA) neurons.
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Specimen
10J:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Hes1tm1Fgu/Hes1tm1Fgu
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
Weak |
Regionally restricted |
10J
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Expression in only a few nuclei localized in mesencephalic dopaminergic (mesDA) neurons.
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Analysis Tools