Antibody:	anti-TH
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS19: midbrain	Present	Regionally restricted	2E	Expression in mesencephalic dopaminergic (mesDA) neurons.

Structure	Level	Pattern	Image	Note
TS19: midbrain	Strong	Regionally restricted	2J	Authors state mesencephalic dopaminergic (mesDA) neurons are more numerous in mutant than in wild type. Expression in mesDA neurons located underneath the midline surface region.

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present	Regionally restricted	3C	Expression in mesencephalic dopaminergic (mesDA) neurons that are distributed perpendicularly, extending processes vertically near the ventricular zone. Expression in relatively tightly fasciculated fibers.

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present	Regionally restricted	3H	Authors state mesencephalic dopaminergic (mesDA) neurons are extended transversely. Expression in neurons widely distributed in midline area of the thinned midbrain.

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present	Regionally restricted	4B	Expression in mesencephalic dopaminergic (mesDA) neurons.

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present	Regionally restricted	4D	Authors state the distribution area of mesencephalic dopaminergic (mesDA) neurons is elongated (extended in the anterior-posterior direction) in the exencephalic brain tissue of the null mutant.

Structure	Level	Pattern	Image	Note
TS21: midbrain	Strong	Regionally restricted	5B	Intense expression in mesencephalic dopaminergic (mesDA) neurons. Expression in ventral tegmental area, oriented perpendicular to ventricular surface, well-developed in the midline. Also expression in mesDA neurons accumulating in a more lateral position, forming the substantia nigra compacta (SNc).

Structure	Level	Pattern	Image	Note
TS21: midbrain	Present	Regionally restricted	5E	Authors state mesencephalic dopaminergic (mesDA) neurons are distributed sparsely at the brain surface. Expression in neurons with short perpendicular extensions.

Structure	Level	Pattern	Image	Note
TS23: midbrain	Present	Regionally restricted	6A	Expression in neurons condensed in the ventral midbrain. Authors report expression in substantia nigra compacta (SNc) and ventral tegmental area (VTA) similar to that of mature animals.

Structure	Level	Pattern	Image	Note
TS23: midbrain	Weak	Regionally restricted	6D	Expression in neurons that are sparsely and widely distributed at the brain surface.

Structure	Level	Pattern	Image	Note
TS23: midbrain	Present	Regionally restricted	7B	Authors state mesencephalic dopaminergic (mesDA) neurons extend the medial forebrain bundle and extend fibers to the striatum. Axons of medial forebrain bundle (mfb) recognizable as fibers running rostrocaudally in lateral margin of hypothalamus. Expression in fibers estending to striatum.

Structure	Level	Pattern	Image	Note
TS23: midbrain	Present	Regionally restricted	7D	In most cases, mesencephalic dopaminergic (mesDA) neurons were loosely distributed in surface region of thinned midbrain. Expression in medial forebrain bundle (mfb), extending fibers to hypothalamus.

Structure	Level	Pattern	Image	Note
TS23: midbrain	Present	Regionally restricted	7F	Authors state projection pathways deviate from those of wild type. In few cases, medial forebrain bundle (mfb) and fibers projecting to striatum do not descend downwards, but run along brain surface.

Structure	Level	Pattern	Image	Note
TS23: midbrain	Present	Regionally restricted	9B	Expression in tightly fasciculated mesencephalic dopaminergic (mesDA) neurons located rostrally close to the midbrain-hindbrain boundary.

Structure	Level	Pattern	Image	Note
TS23: hindbrain	Present	Regionally restricted	9D	Expression in neurons invading the hindbrain. Expression in many neurons distributed in the rostral hindbrain.

Structure	Level	Pattern	Image	Note
TS21: hindbrain	Present	Regionally restricted	9F	Expression in neurons invading the hindbrain. Expression in many neurons distributed in the rostral hindbrain.

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present		10C

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present		10D

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present	Regionally restricted	10E	Expression in mesencephalic dopaminergic (mesDA) neurons.

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present	Regionally restricted	10H	Expression in mesencephalic dopaminergic (mesDA) neurons.