Gene Expression Data
Assay Details
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Assay
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Reference:
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J:23793
Motoyama N, et al., Massive cell death of immature hematopoietic cells and neurons in Bcl-x-deficient mice. Science. 1995 Mar 10;267(5203):1506-10
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6278286
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Gene symbol:
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Map2
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Gene name:
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microtubule-associated protein 2
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Antibody:
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anti-Map2
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Detection system:
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Secondary antibody coupled to Cy3
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Results
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Specimen
2F/H:
(close  )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Bcl2l1tm1Dlo/Bcl2l1tm1Dlo
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: brain ventricular layer |
Weak |
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2F
,
2H
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Little expression.
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| TS20: brainstem |
Strong |
Regionally restricted |
2H
,
2F
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Little expression in the ventricular zone, whereas cells located in the ventral medulla possessed strong expression. The vast majority of degenerating cells, identified by bisbenzamide staining, were located in Map2-positive regions.
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Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Bcl2l1tm1Dlo/Bcl2l1tm1Dlo
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
not shown:
embryonic day 12.5
(more  )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: brain ventricular layer |
Trace |
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Little expression.
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| TS20: spinal cord ventricular layer |
Trace |
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Little expression.
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| TS20: brain |
Strong |
Regionally restricted |
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Cells in the mitotically active ventricular zone lacked immunoreactivity, but that more peripherally located differentiating cells possessed strong staining.
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| TS20: spinal cord lateral wall |
Strong |
Regionally restricted |
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Cells in the mitotically active ventricular zone lacked immunoreactivity, but that more peripherally located differentiating cells possessed strong staining.
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Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Bcl2l1tm1Dlo/Bcl2l1tm1Dlo
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: brain ventricular layer |
Trace |
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Little expression.
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| TS19: neural tube ventricular layer |
Trace |
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Little expression.
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| TS19: brain |
Strong |
Regionally restricted |
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Cells in the mitotically active ventricular zone lacked immunoreactivity, but that more peripherally located differentiating cells possessed strong staining.
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| TS19: neural tube lateral wall |
Strong |
Regionally restricted |
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Cells in the mitotically active ventricular zone lacked immunoreactivity, but that more peripherally located differentiating cells possessed strong staining.
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Specimen
not shown:
embryonic day 11.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: brain ventricular layer |
Trace |
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Little expression.
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| TS19: neural tube ventricular layer |
Trace |
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Little expression.
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| TS19: brain |
Strong |
Regionally restricted |
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Cells in the mitotically active ventricular zone lacked immunoreactivity, but that more peripherally located differentiating cells possessed strong staining.
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| TS19: neural tube lateral wall |
Strong |
Regionally restricted |
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Cells in the mitotically active ventricular zone lacked immunoreactivity, but that more peripherally located differentiating cells possessed strong staining.
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Analysis Tools