Gpc3 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:187541 Schievenbusch S, et al., Neighbor of punc e 11: expression pattern of the new hepatic stem/progenitor cell marker during murine liver development. Stem Cells Dev. 2012 Sep 20;21(14):2656-66
Assay type:	RT-PCR
MGI Accession ID:	MGI:6278165
Gene symbol:	Gpc3
Gene name:	glypican 3

Probe:	Gpc3-pG, Gpc3-pH
Probe preparation:	labelled with SYBR green

Results

Image: 1_Gpc-3

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	83 bp	Amount	Genetic Background	Mutant Allele(s)	Sex
ED11.5	E11.5	TS19: liver	Strong (a)	5 µg; total RNA	C57BL/6		Not Specified
ED12.5	E12.5	TS20: liver	Strong (a)	5 µg; total RNA	C57BL/6		Not Specified
ED13.5	E13.5	TS21: liver	Strong (a)	5 µg; total RNA	C57BL/6		Not Specified
ED14.5	E14.5	TS22: liver	Strong (a)	5 µg; total RNA	C57BL/6		Not Specified
ED15.5	E15.5	TS23: liver	Strong (a)	5 µg; total RNA	C57BL/6		Not Specified
ED16.5	E16.5	TS24: liver	Strong (a)	5 µg; total RNA	C57BL/6		Not Specified
ED17.5	E17.5	TS25: liver	Strong (a)	5 µg; total RNA	C57BL/6		Not Specified
ED18.5	E18.5	TS26: liver	Strong (a)	5 µg; total RNA	C57BL/6		Not Specified
2dpn	P2	TS27: liver	Strong (a)	5 µg; total RNA	C57BL/6		Not Specified
1wpn	P w 1	TS28: liver	Strong (a)	5 µg; total RNA	C57BL/6		Not Specified
2wpn	P w 2	TS28: liver	Present (b)	5 µg; total RNA	C57BL/6		Not Specified
3wpn	P w 3	TS28: liver	Weak (c)	5 µg; total RNA	C57BL/6		Not Specified
AL	P adult	TS28: liver	Trace (d)	5 µg; total RNA	C57BL/6		Not Specified

Notes:
(a) Highly expressed in early stages of liver development. Expression levels 20-fold compared to adult liver.
(b) Expression swiftly declines at 2 wpn. Expression levels 15-fold higher versus adult liver.
(c) Expression further declines to 8-fold higher than adult liver.
(d) Expression barely traceable in adult liver.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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