Probe:	proalpha2(I)
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS17: tail sclerotome	Present	Regionally restricted	1C	Expressed in sclerotomes adjacent to the neural tube and surrounding the notochord.

Structure	Level	Pattern	Image	Note
TS17: head mesenchyme	Present	Regionally restricted	1F/G	Expressed in the cranial mesenchyme surrounding the developing brain.
TS17: dorsal aorta	Present		1F/G
TS17: limb bud	Present		1F/G
TS17: nasal mesenchyme	Present		1F/G
TS17: pericardium	Present		1F/G
TS17: gut mesenchyme	Present	Regionally restricted	1F/G	Expressed in the mesenchyme surrounding the developing intestine.

Structure	Level	Pattern	Image	Note
TS20: atrioventricular endocardial cushion	Present		2B
TS20: dermis	Present		2B
TS20: diaphragm	Present		2B
TS20: mandibular process	Present		2B
TS20: maxillary process	Present		2B
TS20: meninges	Present		2B
TS20: nasal mesenchyme	Present		2B
TS20: tail dermatome	Present		2B
TS20: tail sclerotome	Present		2B
TS20: thoracic body wall	Present		2B
TS20: tongue mesenchyme	Present		2B
TS20: intestine	Present		2B	strongest expression
TS20: umbilical cord	Present		2B	strongest expression
TS20: body wall mesenchyme	Present	Regionally restricted	2B	Expressed in mesenchymal tissue surrounding the rostral prevertebrae.
TS20: pericardium	Present		2B	Also expressed in the fibrous connective tissue.

Structure	Level	Pattern	Image	Note
TS21: diaphragm	Strong		2C
TS21: intestine	Strong		2C
TS21: thoracic body wall	Strong		2C
TS21: umbilical cord	Strong		2C
TS21: tongue mesenchyme	Present	Regionally restricted	2C	Expressed in fibroblasts interspersed between developing muscle fibers.
TS21: nasal mesenchyme	Present		2C
TS21: nasal cavity epithelium	Absent		2C
TS21: tail dermatome	Present	Regionally restricted	2C	segmented expression pattern

Structure	Level	Pattern	Image	Note
TS21: tail dermatome	Present	Regionally restricted	2D	segmented expression pattern

Structure	Level	Pattern	Image	Note
TS22: renal capsule	Present		3D/E	Highest expression.
TS22: renal medullary interstitium	Present		3D/E	Highest expression.

Structure	Level	Pattern	Image	Note
TS25: tibia	Present	Regionally restricted	3F/G	Expressed in the zone of ossification. Expression was absent from proliferating chondrocytes and hypertrophic chondrocytes.
TS25: dermis	Present		3F/G
TS25: perichondrium	Present		3F/G
TS25: periosteum	Present		3F/G
TS25: tendon	Present		3F/G
TS25: epidermis	Absent		3F/G

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Absent
TS11: embryo endoderm	Absent
TS11: embryo mesoderm	Absent

Structure	Level	Pattern	Image	Note
TS13: amnion	Weak
TS13: head mesenchyme	Weak
TS13: pericardium	Weak
TS13: somite	Absent

Structure	Level	Pattern	Image	Note
TS15: pericardium	Present			highest levels of expression
TS15: 1st branchial arch	Present
TS15: forelimb bud	Present
TS15: head mesenchyme	Present

Structure	Level	Pattern	Image	Note
TS17: branchial arch	Present
TS17: dermatome	Present
TS17: sclerotome	Present

Structure	Level	Pattern	Image	Note
TS21: craniocervical region muscle	Present	Regionally restricted		Expressed in fibroblasts interspersed between developing muscle fibers.

Structure	Level	Pattern	Image	Note
TS22: chondrocranium	Present			Highest expression.
TS22: mandible	Present			Highest expression.