Gene Expression Data
Assay Details
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Assay
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Reference:
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J:170532
Pan H, et al., A role for Zic1 and Zic2 in Myf5 regulation and somite myogenesis. Dev Biol. 2011 Mar 1;351(1):120-7
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6274500
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Gene symbol:
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Myf5
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Gene name:
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myogenic factor 5
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Probe:
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Myf5 cDNA13
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1A:
embryonic day 9.5
(more  )
Note: 24 somites
Specimen
1A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Note:
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24 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: dermomyotome |
Present |
Regionally restricted |
1A
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Expression first activated in dorsal medial lip (DML) in E9.5 embryos.
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Specimen
1E:
embryonic day 9.5
(more )
Specimen
1E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: somite |
Present |
Regionally restricted |
1E
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Expression persists in dorsomedial region of somite.
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Specimen
Zic1-/- not shown:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Zic1tm1Jaru/Zic1tm1Jaru
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: somite |
Present |
Regionally restricted |
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Authors report that mutant embryos activate expression normally during somite formation.
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Specimen
Bent Tail not shown:
embryonic day 9.5; Zic3Bn/ Zic3Bn
(more )
Specimen
Bent Tail not shown:
(close )
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Genetic Background:
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BNT/LeJ
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Mutant Allele(s):
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Zic3Bn/Zic3Bn
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: somite |
Present |
Regionally restricted |
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Authors report that mutant embryos activate expression normally during somite formation.
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Specimen
3A:
embryonic day 8.5
(more )
Specimen
3A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Specimen
3B:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Zic2tm1Jaru/Zic2tm1Jaru
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: somite |
Present |
Regionally restricted |
3B
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Authors report expression epaxial activation of expression in posterior somites is delayed at E8.5 and E9.0.
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Specimen
3C:
embryonic day 9.0
(more )
Specimen
3C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Specimen
3D:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Zic2tm1Jaru/Zic2tm1Jaru
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: somite |
Present |
Regionally restricted |
3D
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Activation of expression in epaxial domain is also delayed at E9.5 until approximately the 10th somite pair.
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Specimen
3E:
embryonic day 9.5
(more )
Specimen
3E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Specimen
3F:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Zic2tm1Jaru/Zic2tm1Jaru
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: somite |
Present |
Regionally restricted |
3F
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Authors report delayed activation of expression in epaxial domain.
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Specimen
3G:
embryonic day 9.5
(more )
Specimen
3G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3H:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Zic2tm1Jaru/Zic2tm1Jaru
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: somite |
Present |
Regionally restricted |
3H
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Timing of expression in hypaxial muscle progenitors of ventral lateral lip of myotome is unaffected.
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Specimen
4C:
embryonic day 9.25
(more )
Specimen
4C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.25
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Specimen
not shown Zic1+/-:
embryonic day 9.25; Zic1tm1Jaru/Zic1 +
(more )
Specimen
not shown Zic1+/-:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Zic1tm1Jaru/Zic1+
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Age:
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embryonic day 9.25
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: somite |
Present |
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Authors state expression is unaffected in heterozygous Zic1+/- embryos. embryos.
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Specimen
4D:
embryonic day 9.25; Zic2tm1Jaru/Zic2 +
(more )
Specimen
4D:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Zic2tm1Jaru/Zic2+
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Age:
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embryonic day 9.25
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: somite |
Present |
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4D
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Authors state expression is unaffected in heterozygous Zic2+/kd embryos.
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Specimen
4E:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Zic1tm1Jaru/Zic1+
Zic2tm1Jaru/Zic2+
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Age:
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embryonic day 9.25
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: somite |
Weak |
Regionally restricted |
4E
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Expression is diminished and delayed in the 6th to 9th most posterior somites in compound mutant embryos
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Specimen
4F:
embryonic day 9.5
(more )
Specimen
4F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Specimen
4G:
embryonic day 9.5; Zic2tm1Jaru/Zic2 +
(more )
Specimen
4G:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Zic2tm1Jaru/Zic2+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Specimen
4H:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Zic1tm1Jaru/Zic1+
Zic2tm1Jaru/Zic2+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Specimen
5A Shh+/+:
embryonic day 9.5
(more )
Specimen
5A Shh+/+:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Specimen
5A Shh-/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: somite |
Present |
Regionally restricted |
5A Shh-/-
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No expression in epaxial dorsal medial lip. Expression unaffected in hypaxial ventral lateral lip compared to wild-type embryos.
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Specimen
6E:
embryonic day 9.5
(more )
Specimen
6E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Specimen
6F:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Nogtm1Amc/Nogtm1Amc
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: somite |
Present |
Regionally restricted |
6F
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Activation of expression is delayed in newly formed somites until the 10th somite pair anterior to the presomitic mesoderm.
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