Gene Expression Data
Assay Details
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Assay
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Reference:
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J:165712
Zakin L, et al., Crossveinless-2 is required for the relocalization of Chordin protein within the vertebral field in mouse embryos. Dev Biol. 2010 Nov 1;347(1):204-15
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6273780
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Gene symbol:
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Chrd
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Gene name:
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chordin
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Probe:
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chordin probe2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2C:
embryonic day 12.5
(more  )
Specimen
2C:
(close  )
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Genetic Background:
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involves: 129 * C57BL/6J * SJL/J
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: axial skeleton |
Present |
Regionally restricted |
2C
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Expression in prospective intervertebral discs.
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Specimen
2D:
embryonic day 12.5
(more )
Specimen
2D:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J * SJL/J
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3B/C:
embryonic day 12.5
(more )
Note: Double labeled: blue - Chd (Chrd) RNA; brown - Chd (Chrd) protein (assay MGI:6273775).
Specimen
3B/C:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J * SJL/J
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: blue - Chd (Chrd) RNA; brown - Chd (Chrd) protein (assay MGI:6273775).
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Specimen
S3A:
embryonic day 12.5
(more )
Specimen
S3A:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J * SJL/J
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: axial skeleton |
Present |
Regionally restricted |
S3A
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Expression in prospective intervertebral discs.
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Specimen
S3B:
embryonic day 12.5
(more )
Specimen
S3B:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J * SJL/J
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
S3C:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6J * SJL/J
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Mutant Allele(s):
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Bmpertm1Emdr/Bmpertm1Emdr
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: axial skeleton |
Present |
Regionally restricted |
S3C
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Expression in prospective intervertebral discs.
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Specimen
S3D:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6J * SJL/J
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Mutant Allele(s):
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Bmpertm1Emdr/Bmpertm1Emdr
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: axial skeleton thoracic region |
Present |
Regionally restricted |
S3D
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Expression in prospective intervertebral discs. Intervertebral disc is moderately wider in CV2-/- embryos compared to wild-type; vertebral body is reduced.
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Analysis Tools