Probe:	Barhl1 cDNA2
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent		2A

Structure	Level	Pattern	Image	Note
TS18: diencephalon	Present	Regionally restricted	2B	Expression was detected in the basal plate of the diencephalon, dorsal and ventral thalamus, and the mammillary area.
TS18: forebrain-midbrain boundary region	Present	Regionally restricted	2B	Expression was detected in the alar and caudal region of the pretectum.
TS18: midbrain	Present	Regionally restricted	2B	Expression was detected in the alar plate of the mesencephalon.
TS18: rhombic lip	Present	Regionally restricted	2B	The anterior and posterior rhombic lip was labeled.
TS18: telencephalon	Absent		2B

Structure	Level	Pattern	Image	Note
TS18: future spinal cord	Present	Regionally restricted	2C	Expression was detected in the dorsal alar plate.
TS18: neural tube roof plate	Absent		2C

Structure	Level	Pattern	Image	Note
TS22: inferior colliculus	Present		2D
TS22: mammillary area	Present		2D
TS22: superior colliculus	Present		2D
TS22: cerebellum layer	Present	Regionally restricted	2D	Expression was detected in the external granular cell layer.
TS22: hindbrain	Present	Regionally restricted	2D	Expression was detected in the anterior and posterior extramural migratory stream.

Structure	Level	Pattern	Image	Note
TS22: inferior colliculus	Present		2E
TS22: mammillary area	Present		2E
TS22: superior colliculus	Present		2E
TS22: cerebellum layer	Present	Regionally restricted	2E	Expression was detected in the external granular cell layer.
TS22: hindbrain	Present	Regionally restricted	2E	Expression was detected in the anterior and posterior extramural migratory stream.
TS22: spinal cord	Present	Regionally restricted	2E	Expression was detected in the middle of the spinal cord.

Structure	Level	Pattern	Image	Note
TS18: midbrain	Present	Regionally restricted	3A/B	In the mesencephalon, expression was observed in a subtle cell layer of migrating cells in the cortical plate of the alar plate.

Structure	Level	Pattern	Image	Note
TS18: diencephalon	Present	Regionally restricted	3D/E	Expression was detected in the basal plate of the diencephalon, dorsal and ventral thalamus, and the mammillary area.

Structure	Level	Pattern	Image	Note
TS18: rhombic lip	Present	Regionally restricted	3G/H	In the anterior rhombic lip, expression was particularly noted in the dorsal part of the alar plate corresponding to the progenitor cells of the external granular cell layer.

Structure	Level	Pattern	Image	Note
TS22: superior colliculus	Weak	Regionally restricted	4A/B	Expression was detected at a relatively lower level. Signal was localized in the cortical plate.

Structure	Level	Pattern	Image	Note
TS22: mammillary area	Present		4C/D
TS22: hindbrain	Present	Regionally restricted	4C/D	Expression was detected in the anterior extramural migratory stream.

Structure	Level	Pattern	Image	Note
TS22: cerebellum layer	Strong	Regionally restricted	4E/F	High expression was detected in the external granular cell layer.
TS22: inferior colliculus	Present	Regionally restricted	4E/F	Expression was detected in the dorsal and anterior part of the inferior colliculus.

Structure	Level	Pattern	Image	Note
TS18: future spinal cord	Present	Regionally restricted	5A/B	Expression was in the most dorsal area of the alar plate.
TS18: neural tube roof plate	Absent		5A/B

Structure	Level	Pattern	Image	Note
TS22: spinal cord mantle layer	Present		5D/E	Expression was delimited to the boundary of the mantle zone between the alar and the basal plates.