Gene Expression Data
Assay Details
Assay
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Reference:
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J:266904
Martynova E, et al., Identification of Novel Gata3 Distal Enhancers Active in Mouse Embryonic Lens. Dev Dyn. 2018 Nov;247(11):1186-1198
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:6273344
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Gene symbol:
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Gata3
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Gene name:
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GATA binding protein 3
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Reporter:
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GFP
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Antibody:
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anti-GFP (Invitrogen)
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
1B:
(close )
Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Gata3tm1.2Mbu/Gata3+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1C:
(close )
Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Gata3tm1.2Mbu/Gata3+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS17: lens vesicle |
Strong |
Regionally restricted |
1C
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Expression is at the posterior part.
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Specimen
1D:
(close )
Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Gata3tm1.2Mbu/Gata3+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS20: lens vesicle |
Present |
Regionally restricted |
1D
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Expression is at the transitional area in lens fiber cells.
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Specimen
1E:
(close )
Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Gata3tm1.2Mbu/Gata3+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS22: lens fiber |
Present |
Regionally restricted |
1E
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Expression is at the transitional area.
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Specimen
1F:
(close )
Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Gata3tm1.2Mbu/Gata3+
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS24: lens fiber |
Present |
Regionally restricted |
1F
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Expression is at the transitional area.
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Specimen
Not shown E11.5:
embryonic day 11.5; Gata3tm1.2Mbu/Gata3 +
(more )
Specimen
Not shown E11.5:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd
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Mutant Allele(s):
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Gata3tm1.2Mbu/Gata3+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
Not shown E18.5:
embryonic day 18.5; Gata3tm1.2Mbu/Gata3 +
(more )
Specimen
Not shown E18.5:
(close )
Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Gata3tm1.2Mbu/Gata3+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS26: lens |
Absent |
|
|
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Specimen
Not shown neonate:
postnatal newborn; Gata3tm1.2Mbu/Gata3 +
(more )
Specimen
Not shown neonate:
(close )
Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
|
Gata3tm1.2Mbu/Gata3+
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Age:
|
postnatal newborn
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS27: lens |
Absent |
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