Probe:	PTHrP cDNA5
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS8: trophectoderm	Present		1A/B
TS8: embryo	Absent		1A/B
TS8: epiblast	Absent		1A/B

Structure	Level	Pattern	Image	Note
TS28: endometrium epithelium	Strong	Regionally restricted	1A/B	Expression is in epithelial cells lining the mesometrial crypt marking the implantation site.
TS28: decidua	Present	Regionally restricted	1A/B	Expression is in the outer zone.

Structure	Level	Pattern	Image	Note
TS8: trophectoderm	Present		1C/D
TS8: embryo	Absent		1C/D
TS8: epiblast	Absent		1C/D

Structure	Level	Pattern	Image	Note
TS28: endometrium epithelium	Strong	Regionally restricted	1C/D	Expression is in epithelial cells lining the mesometrial crypt marking the implantation site.
TS28: decidua	Strong	Regionally restricted	1C/D	Expression is in the inner zone of the decidual reaction surrounding the implantation crypt and is weaker in the outer zone.

Structure	Level	Pattern	Image	Note
TS8: trophectoderm	Present		1E/F
TS8: embryo	Absent		1E/F
TS8: epiblast	Absent		1E/F

Structure	Level	Pattern	Image	Note
TS28: endometrium epithelium	Strong	Regionally restricted	1E/F	Expression is in epithelial cells lining the mesometrial crypt marking the implantation site.
TS28: decidua	Present	Regionally restricted	1E/F	Expression is in the outer zone.

Structure	Level	Pattern	Image	Note
TS8: trophectoderm	Present		1G/H
TS8: embryo	Absent		1G/H

Structure	Level	Pattern	Image	Note
TS28: endometrium epithelium	Strong	Regionally restricted	1G/H	Expression is in epithelial cells lining the mesometrial crypt marking the implantation site.
TS28: decidua	Present	Regionally restricted	1G/H	Expression is in the outer zone.

Structure	Level	Pattern	Image	Note
TS8: trophectoderm	Present		1I/J
TS8: embryo	Absent		1I/J
TS8: epiblast	Absent		1I/J

Structure	Level	Pattern	Image	Note
TS28: endometrium epithelium	Strong	Regionally restricted	1I/J	Expression is in epithelial cells lining the mesometrial crypt marking the implantation site.
TS28: decidua	Present	Regionally restricted	1I/J	Expression is in the outer zone.
TS28: myometrium inner circular smooth muscle layer	Present		1I/J
TS28: myometrium outer longitudinal smooth muscle layer	Present		1I/J

Structure	Level	Pattern	Image	Note
TS9: trophectoderm	Present	Regionally restricted	2A/B	Expression is in most trophoblast cells.
TS9: ectoplacental cone	Weak		2A/B
TS9: embryo	Absent		2A/B
TS9: extraembryonic ectoderm	Absent		2A/B

Structure	Level	Pattern	Image	Note
TS28: decidua	Present	Regionally restricted	2A/B	Expression is in the decidual cell layers surrounding the extra-embryonic parts.

Structure	Level	Pattern	Image	Note
TS9: trophectoderm	Present	Regionally restricted	2C/D	Expression is in most trophoblast cells.
TS9: embryo	Absent		2C/D
TS9: embryo endoderm	Absent		2C/D
TS9: extraembryonic ectoderm	Absent		2C/D
TS9: extraembryonic endoderm	Absent		2C/D

Structure	Level	Pattern	Image	Note
TS28: decidua	Present	Regionally restricted	2C/D	Expression is in the decidual cell layers surrounding the extra-embryonic parts.

Structure	Level	Pattern	Image	Note
TS9: trophectoderm	Present	Regionally restricted	2E/F	Expression is in most trophoblast cells.
TS9: embryo	Absent		2E/F
TS9: embryo ectoderm	Absent		2E/F
TS9: embryo endoderm	Absent		2E/F
TS9: extraembryonic endoderm	Absent		2E/F
TS9: trophoblast giant cell	Absent		2E/F

Structure	Level	Pattern	Image	Note
TS28: decidua	Present	Regionally restricted	2E/F	Expression is in the decidual cell layers surrounding the embryonic parts.

Structure	Level	Pattern	Image	Note
TS9: trophectoderm	Present	Regionally restricted	2G/H	Expression is in most trophoblast cells.
TS9: embryo	Absent		2G/H
TS9: embryo ectoderm	Absent		2G/H
TS9: embryo endoderm	Absent		2G/H
TS9: extraembryonic endoderm	Absent		2G/H

Structure	Level	Pattern	Image	Note
TS28: decidua	Present	Regionally restricted	2G/H	Expression is in the decidual cell layers surrounding the embryonic parts.

Structure	Level	Pattern	Image	Note
TS11: trophectoderm	Strong		3A/B
TS11: allantois	Absent		3A/B
TS11: embryo	Absent		3A/B
TS11: parietal endoderm	Absent		3A/B

Structure	Level	Pattern	Image	Note
TS28: decidua	Strong	Single cells	3A/B	Expression is in some of the cells adjacent to the embryo.

Structure	Level	Pattern	Image	Note
TS15: trophoblast giant cell	Present		3E/F , 3C/D
TS15: embryo	Absent		3C/D , 3E/F

Structure	Level	Pattern	Image	Note
TS28: decidua	Strong	Single cells	3E/F , 3C/D	Expression is in scattered cells in the outer zone and is absent in decidual cells neighboring the embryo.
TS28: myometrium inner circular smooth muscle layer	Present	Single cells	3C/D
TS28: myometrium outer longitudinal smooth muscle layer	Present	Single cells	3C/D

Structure	Level	Pattern	Image	Note
TS20: face skin	Strong	Regionally restricted	4A/B	Expression is in the dermis of the snout region.
TS20: choroid plexus	Present		4A/B
TS20: dermis	Present		4A/B
TS20: lung epithelium	Present		4A/B
TS20: mandible primordium	Present		4A/B
TS20: rib pre-cartilage condensation	Present		4A/B
TS20: vertebral pre-cartilage condensation	Present		4A/B
TS20: nasal mesenchyme	Strong	Regionally restricted	4A/B	Expression is in the mesenchyme lining the nasal pit.
TS20: heart ventricle	Present	Regionally restricted	4A/B	Expression is in some epithelial cells.
TS20: bone	Present	Regionally restricted	4A/B	Expression is at all sites of endochondrial bone formation including the bones of the extremeties.
TS20: skeleton	Present	Regionally restricted	4A/B	Expression is at all sites of endochondrial bone formation including the bones of the extremeties.
TS20: inner ear	Present	Regionally restricted	4A/B	Expression is in the semilateral channels.

Structure	Level	Pattern	Image	Note
TS20: face skin	Strong	Regionally restricted	4C/D	Expression is in the dermis of the snout region.
TS20: choroid plexus	Present		4C/D
TS20: dermis	Present		4C/D
TS20: lung epithelium	Present		4C/D
TS20: mandible primordium	Present		4C/D
TS20: rib pre-cartilage condensation	Present		4C/D
TS20: vertebral pre-cartilage condensation	Present		4C/D
TS20: nasal mesenchyme	Strong	Regionally restricted	4C/D	Expression is in the mesenchyme lining the nasal pit.
TS20: heart ventricle	Present	Regionally restricted	4C/D	Expression is in some epithelial cells.
TS20: bone	Present	Regionally restricted	4C/D	Expression is at all sites of endochondrial bone formation including the bones of the extremeties.
TS20: inner ear	Present	Regionally restricted	4C/D	Expression is in the semilateral channels.

Structure	Level	Pattern	Image	Note
TS20: hindlimb pre-cartilage condensation	Present	Regionally restricted	5A/B	Expression is in the growth plate chondrocytes and is absent in the maturing chondrocytes.
TS20: dermis	Strong	Clusters	5A/B	Expression is in two small clusters rostrally and caudally relative to the hindlimb bud.

Structure	Level	Pattern	Image	Note
TS20: forelimb pre-cartilage condensation	Present	Regionally restricted	5C/D	Expression is in the growth plate chondrocytes and is absent in the maturing chondrocytes.
TS20: dermis	Strong	Regionally restricted	5C/D	Expression is in two small clusters rostrally and caudally relative to the forelimb bud.

Structure	Level	Pattern	Image	Note
TS20: 4th ventricle choroid plexus	Present		6A/B
TS20: diencephalon roof plate	Present		6A/B
TS20: lateral ventricle choroid plexus	Present		6A/B

Structure	Level	Pattern	Image	Note
TS20: choroid invagination	Present	Regionally restricted	6C/D	Expression is in the choroid fissure.
TS20: choroid plexus	Present		6C/D

Structure	Level	Pattern	Image	Note
TS20: choroid plexus	Present		6E/F

Structure	Level	Pattern	Image	Note
TS20: choroid plexus	Present		6G/H

Structure	Level	Pattern	Image	Note
TS20: semicircular canal	Present		6I/J
TS20: endolymphatic sac	Absent		6I/J

Structure	Level	Pattern	Image	Note
TS20: Meckel's cartilage	Strong		6K/L
TS20: otic capsule	Strong		6K/L

Structure	Level	Pattern	Image	Note
TS13: trophoblast giant cell	Present
TS13: embryo	Absent

Structure	Level	Pattern	Image	Note
TS28: myometrium inner circular smooth muscle layer	Present
TS28: myometrium outer longitudinal smooth muscle layer	Present

Structure	Level	Pattern	Image	Note
TS17: hindbrain roof plate	Weak

Structure	Level	Pattern	Image	Note
TS19: hindbrain roof plate	Present
TS19: otocyst epithelium	Present
TS19: rib pre-cartilage condensation	Present
TS19: trophectoderm	Present
TS19: vertebral pre-cartilage condensation	Present