Gene Expression Data
Assay Details
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Assay
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Reference:
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J:154906
Wang W, et al., Atf4 regulates chondrocyte proliferation and differentiation during endochondral ossification by activating Ihh transcription. Development. 2009 Dec;136(24):4143-53
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6273222
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Gene symbol:
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Pth1r
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Gene name:
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parathyroid hormone 1 receptor
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Probe:
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PPR probe2
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Probe preparation:
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Antisense labelled with S35 RNA
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Results
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Specimen
5Ag:
embryonic day 14.5
(more  )
Specimen
5Ag:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5Ag':
(close )
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Genetic Background:
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involves: 129
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Mutant Allele(s):
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Atf4tm1Tow/Atf4tm1Tow
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: humerus cartilage condensation |
Present |
Regionally restricted |
5Ag'
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Authors report normal expression in Atf-/- growth plates. Similar expression levels in wild type and Atf4-/- humeri.
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Specimen
5Ah:
embryonic day 16.5
(more )
Specimen
5Ah:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: humerus |
Present |
Regionally restricted |
5Ah
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Expression transiently expanded to cells of periosteum and to osteoblasts at E16.
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Specimen
5Ah':
(close )
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Genetic Background:
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involves: 129
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Mutant Allele(s):
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Atf4tm1Tow/Atf4tm1Tow
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: humerus |
Present |
Regionally restricted |
5Ah'
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Authors report normal expression in Atf-/- growth plates. Similar expression levels in wild type and Atf4-/- humeri. Expression transiently expanded to cells of periosteum and to osteoblasts at E16.
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Specimen
5Ai:
postnatal day 0
(more )
Specimen
5Ai:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: humerus |
Present |
Regionally restricted |
5Ai
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Expression diminished in osteoblasts at birth in both wildtype and Atf-/- humeri.
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Specimen
5Ai':
(close )
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Genetic Background:
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involves: 129
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Mutant Allele(s):
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Atf4tm1Tow/Atf4tm1Tow
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: humerus |
Present |
Regionally restricted |
5Ai'
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Authors report normal expression in Atf-/- growth plates. Similar expression levels in wild type and Atf4-/- humeri. Expression diminished in osteoblasts at birth in both wildtype and Atf-/- humeri.
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Analysis Tools