Summary | Transgene origin | Transgene description | Find Mice (IMSR) | References

Symbol:	Tg(ACTA1-ATP2A1*/DsRed/GFP)5Wheel
Name:	transgene insertion 5, Thurman Wheeler
MGI ID:	MGI:6272824
Synonyms:	Therapy Reporter, TR
Transgene:	Tg(ACTA1-ATP2A1*/DsRed/GFP)5Wheel  Location: unknown
Alliance:	Tg(ACTA1-ATP2A1*/DsRed/GFP)5Wheel page

Strain of Origin:

FVB/N

Transgene Type:    Transgenic (Humanized sequence, Reporter) Mutation:    Insertion   Mutation details: The transgenic construct is designed with a human skeletal actin (ACTA1 or HSA) promoter followed by a human ATP2A1 exon 22 minigene with a single base deletion to reduce the number of base pairs from 42 to 41. The deletion allows transcription to bypass a stop codon and shift the reading frame. The ATP2A1 minigene is followed by sequence from a DsRed reporter fused to a GFP reporter. If alternative splicing includes exon 22 then the DsRed sequence is in-frame; if exon 22 is skipped then the reading frame shifts to GFP. The bichromatic shift occurs because DsRed has two open reading frames. (J:267578)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:267578 Hu N, et al., Non-invasive monitoring of alternative splicing outcomes to identify candidate therapies for myotonic dystrophy type 1. Nat Commun. 2018 Dec 7;9(1):5227
All:	1 reference(s)