Reporter:	GFP
Antibody:	anti-GFP (ab13970)
Detection system:	Secondary antibody coupled to Alexa Fluor

Structure	Level	Pattern	Image	Note
TS27: retina ganglion cell layer	Present		3B +/- EGFP , 3B +/- merge	Pax6-positive cells mostly express EGFP at the appropriate intensities, while some of them express weaker/stronger EGFP than the endogenous levels.
TS27: retina inner nuclear layer	Present		3B +/- EGFP , 3B +/- merge	Pax6-positive cells mostly express EGFP at the appropriate intensities, while some of them express weaker/stronger EGFP than the endogenous levels.

Structure	Level	Pattern	Image	Note
TS27: retina ganglion cell layer	Present		3B -/- EGFP , 3B -/- merge	EGFP expressions precisely recapitulated the endogenous expression profiles.
TS27: retina inner nuclear layer	Present		3B -/- EGFP , 3B -/- merge	EGFP expressions precisely recapitulated the endogenous expression profiles.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present	Graded	4B upper right	EGFP expressions were mediolaterally graded, being most intense in the lateral side, and were totally comparable to the endogenous expression pattern.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present	Graded	4B lower right	EGFP expressions were rostrocaudally graded, being most thick in the rostral side, recapitulating the endogenous expressions.

Structure	Level	Pattern	Image	Note
TS27: retina ganglion cell layer	Present		S3B upper middle , S3A upper , S3B upper right	Approximately half of Pax6-positive cells within GCL expressed EGFP at the equivalent intensities, while the others expressed weaker/stronger EGFP than the endogenous Pax6.
TS27: retina inner nuclear layer	Present		S3B upper middle , S3B upper right , S3A upper	More than 70% of Pax6-positive cells expressed EGFP at the appropriate levels.

Structure	Level	Pattern	Image	Note
TS27: retina ganglion cell layer	Present		S3A lower , S3B lower right , S3B lower middle	Nearly all the Pax6-positive cells both in GCL and INL expressed EGFP at the comparable intensities, more accurately recapitulating the endogenous expression profiles.
TS27: retina inner nuclear layer	Present		S3A lower , S3B lower right , S3B lower middle	Nearly all the Pax6-positive cells both in GCL and INL expressed EGFP at the comparable intensities, more accurately recapitulating the endogenous expression profiles.

Structure	Level	Pattern	Image	Note
TS27: retina ganglion cell layer	Present		S4A middle , S4A right	Expressed in most cells. Pax6 and EGFP expressed at the similar levels.
TS27: retina inner nuclear layer	Present		S4A middle , S4A right	Expressed in most cells. Pax6 and EGFP expressed at the similar levels.

Structure	Level	Pattern	Image	Note
TS28: testis	Present		S4B middle , S4B right	EGFP expressions in testis substantially overlap with the Pax6 expressions.