Probe:	P5
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	Probes were hydrolyzed by alkaline hydrolysis to an average length of 150 nucleotides.

Structure	Level	Pattern	Image	Note
TS11: future brain neural fold	Present		2a/a'

Structure	Level	Pattern	Image	Note
TS12: neural ectoderm	Strong		2b/b'

Structure	Level	Pattern	Image	Note
TS13: allantois	Present		2c/c'
TS13: foregut diverticulum endoderm	Present		2c/c'
TS13: neural ectoderm	Present		2c/c'
TS13: embryo endoderm	Present	Regionally restricted	2c/c'	Including the endodermal component of the heart pocket.

Structure	Level	Pattern	Image	Note
TS13: future brain	Present		2d/d'

Structure	Level	Pattern	Image	Note
TS13: foregut diverticulum endoderm	Present		2e/e'
TS13: future brain	Present		2e/e'

Structure	Level	Pattern	Image	Note
TS13: foregut diverticulum endoderm	Present		2f/f'
TS13: neural ectoderm	Present		2f/f'

Structure	Level	Pattern	Image	Note
TS15: neural ectoderm	Strong		3a/a'
TS15: hindgut	Present		3a/a'

Structure	Level	Pattern	Image	Note
TS15: branchial arch ectoderm	Present		3b/b'
TS15: branchial arch endoderm	Present		3b/b'
TS15: branchial arch mesenchyme	Present		3b/b'
TS15: fronto-nasal process mesenchyme	Present		3b/b'
TS15: olfactory placode	Present		3b/b'
TS15: otocyst	Present		3b/b'

Structure	Level	Pattern	Image	Note
TS15: forelimb bud	Present		3c/c'
TS15: hindgut	Present		3c/c'

Structure	Level	Pattern	Image	Note
TS15: neural ectoderm	Strong		3d/d'
TS15: branchial arch ectoderm	Present		3d/d'
TS15: branchial arch endoderm	Present		3d/d'
TS15: branchial arch mesenchyme	Present		3d/d'
TS15: optic stalk	Present		3d/d'
TS15: optic vesicle	Present		3d/d'

Structure	Level	Pattern	Image	Note
TS15: neural ectoderm	Strong		3e/e'
TS15: otocyst	Present		3e/e'

Structure	Level	Pattern	Image	Note
TS17: midbrain	Present		3g/g'
TS17: midbrain mantle layer	Weak	Regionally restricted	3g/g'	Expression is in the basal midbrain.
TS17: diencephalon	Weak	Regionally restricted	3g/g'	Expression is in the regions of the tuberal and optic stalk of the hypothalamus.

Structure	Level	Pattern	Image	Note
TS17: central nervous system	Strong		3h/h'
TS17: branchial arch mesenchyme	Weak		3h/h'

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present		3i/i'
TS17: diencephalon	Weak	Regionally restricted	3i/i'	Expression is in the regions of the tuberal and optic stalk of the hypothalamus.

Structure	Level	Pattern	Image	Note
TS17: central nervous system	Present		3j/j'
TS17: hindbrain floor plate	Weak		3j/j'

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present		3k/k'
TS17: hindbrain floor plate	Weak		3k/k'

Structure	Level	Pattern	Image	Note
TS16: future spinal cord	Present	Regionally restricted	4a/a'	There is ubiquitous expression except in the basal mantle layer and floor plate.
TS16: neural tube floor plate	Absent		4a/a'

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	4b/b'	There is ubiquitous expression except in the basal mantle layer and floor plate.
TS20: basal columns	Present		4b/b'
TS20: alar columns	Present		4b/b'	The ventral border corresponds to the sulcus limitans.
TS20: spinal cord mantle layer	Present	Regionally restricted	4b/b'	Expression is absent in the basal region.
TS20: spinal cord floor plate	Absent		4b/b'

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Regionally restricted	4c/c'	There is ubiquitous expression except in the basal mantle layer and floor plate.
TS21: spinal cord mantle layer	Present	Regionally restricted	4c/c'	Expression is absent in the basal region.
TS21: spinal cord floor plate	Absent		4c/c'

Structure	Level	Pattern	Image	Note
TS22: spinal cord	Present	Regionally restricted	4d/d'	There is ubiquitous expression except in the basal mantle layer and floor plate.
TS22: spinal cord mantle layer	Present	Regionally restricted	4d/d'	Expression is absent in the basal region.
TS22: spinal cord floor plate	Absent		4d/d'

Structure	Level	Pattern	Image	Note
TS24: alar columns	Strong	Regionally restricted	4e/e'	Expression is in a subregion of the somatic-sensory area.
TS24: basal columns	Strong		4e/e'
TS24: intermediate grey horn	Strong	Regionally restricted	4e/e'	Expression is in two large spots in the visceral-sensory area of the lateral grey horn.
TS24: spinal cord	Present	Regionally restricted	4e/e'
TS24: spinal cord mantle layer	Present	Regionally restricted	4e/e'
TS24: spinal cord floor plate	Absent		4e/e'

Structure	Level	Pattern	Image	Note
TS20: epithalamus	Present		5a/a'
TS20: preoptic area	Present		5a/a'
TS20: thalamus	Present	Regionally restricted	5a/a'	Expression is in the alarmost part of the dorsal thalamus.
TS20: forebrain-midbrain boundary region	Present	Regionally restricted	5a/a'	Expression is in a large stripe corresponding to the anterior pretectal area and is weak in the posterior pretectal region.
TS20: liver	Weak		5a/a'
TS20: posterior commissure	Weak		5a/a'
TS20: hypothalamus	Present	Regionally restricted	5a/a'	Expression is in the eminentia thalami and the surpaoptic paraventricular region.

Structure	Level	Pattern	Image	Note
TS20: Meckel's cartilage	Present		5b/b'
TS20: epithalamus	Present		5b/b'
TS20: mammillary area	Present		5b/b'
TS20: mandibular process	Present		5b/b'
TS20: tongue muscle	Present		5b/b'
TS20: thalamus	Present	Regionally restricted	5b/b'	Expression is in the alarmost part of the dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS20: cerebellum primordium	Present		5c/c'
TS20: duodenum	Present		5c/c'
TS20: epithalamus	Present		5c/c'
TS20: hindbrain	Present		5c/c'
TS20: mammillary area	Present		5c/c'
TS20: midbrain roof plate	Present		5c/c'
TS20: midgut loop	Present		5c/c'
TS20: spinal cord	Present		5c/c'
TS20: vertebral cartilage condensation	Present		5c/c'
TS20: midbrain	Present	Regionally restricted	5c/c'	Expression is in the alarmost region.
TS20: thalamus	Present	Regionally restricted	5c/c'	Expression is in the alarmost part of the dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS20: hindbrain alar plate	Present	Regionally restricted	5d/d'	Expression is in the germinal layer with an increase at the level of the sulcus limitans.
TS20: hindbrain basal plate	Present	Regionally restricted	5d/d'	Expression is in the germinal layer with an increase at the level of the sulcus limitans.
TS20: thalamus	Present	Regionally restricted	5d/d'	Expression is in the alarmost part of the dorsal thalamus.
TS20: epithalamus	Present		5d/d'
TS20: mammillary area	Present		5d/d'
TS20: preoptic area	Present		5d/d'
TS20: forebrain-midbrain boundary region	Present	Regionally restricted	5d/d'	Expression is in a large stripe corresponding to the anterior pretectal area and is weak in the posterior pretectal region.
TS20: hypothalamus	Present	Regionally restricted	5d/d'	Expression is in the eminentia thalami and the surpaoptic paraventricular region.
TS20: posterior commissure	Weak		5d/d'

Structure	Level	Pattern	Image	Note
TS20: hindbrain alar plate	Present	Regionally restricted	5e/e'	Expression is in the germinal layer with an increase at the level of the sulcus limitans.
TS20: hindbrain basal plate	Present	Regionally restricted	5e/e'	Expression is in the germinal layer with an increase at the level of the sulcus limitans.
TS20: forebrain-midbrain boundary region	Present	Regionally restricted	5e/e'	Expression is in a large stripe corresponding to the anterior pretectal area and is weak in the posterior pretectal region.
TS20: hypothalamus	Present	Regionally restricted	5e/e'	Expression is in the eminentia thalami and the surpaoptic paraventricular region.
TS20: mammillary area	Present		5e/e'
TS20: preoptic area	Present		5e/e'
TS20: posterior commissure	Weak		5e/e'

Structure	Level	Pattern	Image	Note
TS20: forebrain-midbrain boundary region	Present	Regionally restricted	5f/f'	Expression is in a large stripe corresponding to the anterior pretectal area and is weak in the posterior pretectal region.
TS20: hypothalamus	Present	Regionally restricted	5f/f'	Expression is in the eminentia thalami and the surpaoptic paraventricular region.
TS20: mammillary area	Present		5f/f'
TS20: neurohypophysis infundibulum	Present		5f/f'
TS20: preoptic area	Present		5f/f'
TS20: posterior commissure	Weak		5f/f'

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	5g/g'	Expression is in the septal area.

Structure	Level	Pattern	Image	Note
TS20: duodenum	Present		5h/h'
TS20: epithalamus	Present		5h/h'
TS20: midgut loop	Present		5h/h'
TS20: thalamus	Present	Regionally restricted	5h/h'	Expression is in the alarmost part of the dorsal thalamus.
TS20: liver	Weak		5h/h'

Structure	Level	Pattern	Image	Note
TS20: forebrain-midbrain boundary region	Present	Regionally restricted	5i/i'	Expression is in a large stripe corresponding to the anterior pretectal area and is weak in the posterior pretectal region.
TS20: stomach	Present	Regionally restricted	5i/i'	Expression is in the mucosal layer.
TS20: duodenum	Present		5i/i'
TS20: midgut loop	Present		5i/i'
TS20: liver	Weak		5i/i'
TS20: posterior commissure	Weak		5i/i'

Structure	Level	Pattern	Image	Note
TS20: cerebellum primordium	Present		5j/j'
TS20: midbrain roof plate	Present		5j/j'
TS20: midbrain	Present	Regionally restricted	5j/j'	Expression is in the alarmost region.
TS20: liver	Weak		5j/j'

Structure	Level	Pattern	Image	Note
TS16: optic cup	Present	Regionally restricted	6a/a'	Expression is in the retina.
TS16: lens pit	Absent		6a/a'

Structure	Level	Pattern	Image	Note
TS20: neural retina	Present		6b/b'

Structure	Level	Pattern	Image	Note
TS22: retina	Present	Regionally restricted	6c/c'	Expression is in close proximity to the future ciliary body.

Structure	Level	Pattern	Image	Note
TS16: otocyst	Present	Regionally restricted	6d/d'	Expression gradually increases along the mediolateral diameter.

Structure	Level	Pattern	Image	Note
TS20: inner ear epithelium	Present	Regionally restricted	6e/e'	Expression is in the basal epithelium.

Structure	Level	Pattern	Image	Note
TS20: neurohypophysis infundibulum	Present		6f/f'

Structure	Level	Pattern	Image	Note
TS24: adenohypophysis	Present		6g/g'
TS24: neurohypophysis	Present		6g/g'

Structure	Level	Pattern	Image	Note
TS21: adrenal gland	Present		6h/h'

Structure	Level	Pattern	Image	Note
TS24: adrenal gland	Present		6i/i'

Structure	Level	Pattern	Image	Note
TS21: epithalamus	Present		7a/a'
TS21: zona limitans intrathalamica	Present		7a/a'
TS21: thalamus	Present	Regionally restricted	7a/a'	Expression is in the dorsal thalamus.
TS21: forebrain-midbrain boundary region	Present	Regionally restricted	7a/a'	Expression is in the anterior and posterior pretectum.

Structure	Level	Pattern	Image	Note
TS21: epithalamus	Present		7b/b'
TS21: neurohypophysis infundibulum	Present		7b/b'
TS21: thalamus	Present	Regionally restricted	7b/b'	Expression is in the dorsal thalamus.
TS21: forebrain-midbrain boundary region	Present	Regionally restricted	7b/b'	Expression is in the anterior and posterior pretectum.
TS21: hypothalamus	Present	Regionally restricted	7b/b'	Expression is in restricted areas including the basal tubural area.

Structure	Level	Pattern	Image	Note
TS21: epithalamus	Present		7c
TS21: zona limitans intrathalamica	Present		7c
TS21: thalamus	Present	Regionally restricted	7c	Expression is in the dorsal thalamus.
TS21: forebrain-midbrain boundary region	Present	Regionally restricted	7c	Expression is in the anterior and posterior pretectum.

Structure	Level	Pattern	Image	Note
TS23: zona limitans intrathalamica	Present		7e/e'
TS23: pretectum	Present	Regionally restricted	7e/e'	Expression is as a segment-like domain in the anterior pretectal area.

Structure	Level	Pattern	Image	Note
TS24: dorsal thalamus	Present	Regionally restricted	8a/a'
TS24: pretectum	Present	Regionally restricted	8a/a'	Expression is in the anterior pretectal area.

Structure	Level	Pattern	Image	Note
TS24: pretectum	Present	Regionally restricted	8b/b'	Expression is in the anterior pretectal area.

Structure	Level	Pattern	Image	Note
TS24: dorsal thalamus	Present	Regionally restricted	8c/c'	Including the ventrolateral nucleus.
TS24: thalamus	Present	Regionally restricted	8c/c'	Expression is in the ventral thalamus.
TS24: epithalamus	Present		8c/c'
TS24: medial habenular nucleus	Present		8c/c'
TS24: hippocampus	Present	Regionally restricted	8c/c'	Expression is in the cortical layer.
TS24: hypothalamus	Present		8c/c'	Including the paraventricular and periventricular nuclei.

Structure	Level	Pattern	Image	Note
TS24: pretectum	Present	Regionally restricted	8d/d'	Expression is in the anterior pretectal area.
TS24: thalamus	Present	Regionally restricted	8d/d'	Expression is in the ventral thalamus and the lamina medullaris externa.
TS24: hippocampus	Present	Regionally restricted	8d/d'	Expression is in the cortical layer.
TS24: epithalamus	Present		8d/d'
TS24: reticular thalamic nucleus	Present		8d/d'
TS24: zona incerta	Present		8d/d'
TS24: hypothalamus	Present		8d/d'	Including the paraventricular and periventricular nuclei.

Structure	Level	Pattern	Image	Note
TS20: forebrain-midbrain boundary region	Present	Regionally restricted	9d/d'	Expression is in a large stripe corresponding to the anterior pretectal area and is weak in the posterior pretectal region.
TS20: diencephalon	Present	Regionally restricted	9d/d'	Expression is in a narrow stripe running between the mammillary region, the ventral thalamus, the hypothalamic cell cord, the entopeduncular area, and the dorsal tuberal area.
TS20: hypothalamus	Present		9d/d'	Expression is in a longitudinal area corresponding to the supraoptic/paraventricular area, the anterior hypothalamic area, and the preoptic posterior area.
TS20: thalamus	Present		9d/d'
TS20: posterior commissure	Weak		9d/d'

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present	Regionally restricted	9h/h'	Expression is anterior.

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	9l/l'	Expression is in the external germinal layer of the ventral thalamus.

Structure	Level	Pattern	Image	Note
TS21: hindbrain	Present
TS21: thymus primordium	Present
TS21: stomach	Present			Expression is stronger in the mucosal layer.
TS21: embryo	Present	Regionally restricted		The authors state that the overall expression pattern seen at E12.5 was unaltered in E13.5.

Structure	Level	Pattern	Image	Note
TS22: embryo	Present	Regionally restricted		The authors state that the overall expression pattern seen at E12.5 was unaltered in E14.5.

Structure	Level	Pattern	Image	Note
TS23: embryo	Present	Regionally restricted		The authors state that the overall expression pattern seen at E12.5 was unaltered in E15.

Structure	Level	Pattern	Image	Note
TS23: liver	Strong
TS23: retina	Present	Regionally restricted		Expression is in close proximity to the future ciliary body.

Structure	Level	Pattern	Image	Note
TS24: hindbrain alar plate	Weak
TS24: hindbrain basal plate	Weak
TS24: thymus	Present
TS24: thyroid gland	Present