Antibody:	anti-DCL
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS18: diencephalon	Strong	Widespread	5A	Expression was found throughout the telencephalon and diencephalon. Expression was found in the outer layers close to the pia as well as in the inner ventricular zone.
TS18: telencephalon	Strong	Widespread	5A	Expression was found throughout the telencephalon and diencephalon. Expression was found in the outer layers close to the pia as well as in the inner ventricular zone.
TS18: diencephalon ventricular layer	Present		5A
TS18: telencephalon ventricular layer	Present		5A
TS18: 1st branchial arch mandibular component	Absent		5A

Structure	Level	Pattern	Image	Note
TS16: diencephalon	Strong
TS16: dorsal root ganglion	Strong
TS16: telencephalon	Strong

Structure	Level	Pattern	Image	Note
TS18: dorsal root ganglion	Strong
TS18: sympathetic ganglion	Strong

Structure	Level	Pattern	Image	Note
TS14: central nervous system	Present	Regionally restricted	5C	Expression was abundant in the inner ependymal and outer early preplate-marginal zone region.

Structure	Level	Pattern	Image	Note
TS18: neural tube	Present	Regionally restricted	5D	Expression was abundant at the luminal border, while in the developing neuronal tissue, various mitotic cells were immunopositive.

Structure	Level	Pattern	Image	Note
TS18: telencephalon ventricular layer	Present	Regionally restricted	5E	A mitotic cell was immunopositive in the neocortical ventricular zone.

Structure	Level	Pattern	Image	Note
TS16: telencephalon ventricular layer	Present		5F
TS16: telencephalon	Present	Regionally restricted	5F	Expression was detected in the neuroepithelium and ventricular zone. Many immunopositive processes extended radially towards the pial surface. In the preplate expression was also found in tangentially orientated processes. Mitotic cells were visible in the intermediate zone.

Structure	Level	Pattern	Image	Note
TS14: future telencephalon	Strong	Regionally restricted	5I	Expression was strong in the ventricular zone-ependymal layer, with immunopositive radial processes extending towards the intermediate zone.

Structure	Level	Pattern	Image	Note
TS14: future telencephalon	Present	Regionally restricted	5J	Immunopositive radial processes extended into the subventricular zone and intermediate zone.

Structure	Level	Pattern	Image	Note
TS14: future telencephalon	Present	Regionally restricted	5K	Expression was detected in dividing cells in the ventricular zone.

Structure	Level	Pattern	Image	Note
TS18: telencephalon ventricular layer	Present		5L