Gt(ROSA)26Sortm65.2(CAG-tdTomato)Hze
Targeted Allele Detail
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Symbol: |
Gt(ROSA)26Sortm65.2(CAG-tdTomato)Hze |
Name: |
gene trap ROSA 26, Philippe Soriano; targeted mutation 65.2, Hongkui Zeng |
MGI ID: |
MGI:6260212 |
Synonyms: |
Ai65F, Ai65F(RCF-tdT), fSf-Tomato, R26-frt.Stop.frt-tdTomato, Rosa26-tdT-FRT |
Gene: |
Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
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Alliance: |
Gt(ROSA)26Sortm65.2(CAG-tdTomato)Hze page
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Allele Type: |
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Targeted (Conditional ready, Reporter) |
Mutation: |
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Insertion
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Mutation details: The targeting construct contains (from 5' to 3'): a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), a frt-flanked STOP cassette (with a short open reading frame, followed by translational stops in all three reading frames, a synthetic polyA sequence, a polyA sequence from the human growth hormone gene, and a polyA sequence from the Herpes Simplex Virus TK gene), a loxP-flanked STOP cassette (with stop codons in all three reading frames linked to three repeats of the SV40 polyA signal), a sequence encoding the tdTomato fluorescent protein (a non-oligomerizing DsRed fluorescent protein variant with a 12 residue linker fusing two copies of the protein [tandem dimer]), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a bovine growth hormone polyA signal and an AttL site.This entire targeting vector was inserted between exons 1 and 2. PhiC31-mediated recombination removed the AttB/AttP-flanked PGK-Neo-polyA cassette and replace it with the recombined AttB/AttP site (AttL). This entire targeting vector was inserted between exons 1 and 2. PhiC31-mediated recombination removed the AttB/AttP-flanked PGK-Neo-polyA cassette and replaced it with the recombined AttB/AttP site (AttL). Cre-mediated recombination removed the floxed STOP.
(J:260362)
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Original: |
J:219930 Madisen L, et al., Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Neuron. 2015 Mar 4;85(5):942-58 |
All: |
20 reference(s) |
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