About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:238419 Wang C, et al., Ascl2 inhibits myogenesis by antagonizing the transcriptional activity of myogenic regulatory factors. Development. 2017 Jan 15;144(2):235-247
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6259630
Gene symbol: Ascl2
Gene name: achaete-scute family bHLH transcription factor 2
Antibody: anti-Ascl2 (MAB4418)
Detection system: Secondary antibody
Results
Specimen 1C: embryonic day 12.5 (more )
Note: Triple labeled: green - Ascl2; red - Pax7 (assay MGI:6259631); blue - MyoD1 (assay MGI:6259632).
Structure Level Pattern Image Note
TS20: myotome Present Regionally restricted 1Ca Ascl2 , 1Cb Ascl2 , 1C Expression is in the epaxial myotome.
Specimen 1D top: embryonic day 17.5 (more )
Note: Double labeled: green - Ascl2; red - Pax7 (assay MGI:6259631).
Structure Level Pattern Image Note
TS25: back musculature Present 1D merge top , 1D Asc2 top
Specimen 1D bottom: embryonic day 17.5 (more )
Note: Double labeled: green - Ascl2; red - MyoD1 (assay MGI:6259632).
Structure Level Pattern Image Note
TS25: back musculature Present 1D merge borrom , 1D Ascl2 bottom
Specimen 3A E12.5: embryonic day 12.5 (more )
Note: Double labeled: red - Ascl2; green - Mki67 (assay MGI:6269994).
Structure Level Pattern Image Note
TS20: myotome Present 3A E12.5 merge , 3A E12.5 Ascl2
Specimen 3A E17.5: embryonic day 17.5 (more )
Note: Double labeled: red - Ascl2; green - Mki67 (assay MGI:6269994).
Structure Level Pattern Image Note
TS25: back musculature Present 3A E17.5 Ascl2 , 3A E17.5 merge
Specimen S1B E10.5 not shown: embryonic day 10.5 (more )
Note: These data are shown as a graph of the quantification of the percentage of Ascl2-positive cells in Pax7-positive cells. Data for Pax7 are shown in assay MGI:6259631).
Structure Level Pattern Image Note
TS17: myotome Absent
Specimen S1B E12.5 not shown: embryonic day 12.5 (more )
Note: These data are shown as a graph of the quantification of the percentage of Ascl2-positive cells in Pax7-positive cells. Data for Pax7 are shown in assay MGI:6259631).
Structure Level Pattern Image Note
TS20: myotome Present
Specimen S1B E17.5 not shown: embryonic day 17.5 (more )
Note: These data are shown as a graph of the quantification of the percentage of Ascl2-positive cells in Pax7-positive cells. Data for Pax7 are shown in assay MGI:6259631).
Structure Level Pattern Image Note
TS25: back musculature Present
Specimen S1B P1 not shown: postnatal day 1 (more )
Note: These data are shown as a graph of the quantification of the percentage of Ascl2-positive cells in Pax7-positive cells. Data for Pax7 are shown in assay MGI:6259631).
Structure Level Pattern Image Note
TS27: tibialis anterior Present
Specimen S1B P60 not shown: postnatal day 60 (more )
Note: These data are shown as a graph of the quantification of the percentage of Ascl2-positive cells in Pax7-positive cells. Data for Pax7 are shown in assay MGI:6259631).
Structure Level Pattern Image Note
TS28: tibialis anterior Absent
Specimen S1C: embryonic day 17.5 (more )
Note: Double labeled: red - Ascl2; green - Pax7 (assay MGI:6259631).
Structure Level Pattern Image Note
TS25: back musculature Present S1C Ascl2 , S1C merge
Specimen S1D: embryonic day 17.5 (more )
Note: Triple labeled: red - Ascl2; green - Pax7 (assay MGI:6259631); blue - MyoD1 (assay MGI:6259632).
Structure Level Pattern Image Note
TS25: back musculature Present S1D merge , S1D Ascl2
Specimen S1E: postnatal day 1 (more )
Note: Double labeled: red - Ascl2; green - Pax7 (assay MGI:6259631).
Structure Level Pattern Image Note
TS27: tibialis anterior Present S1E merge , S1E Ascl2
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory