Antibody:	anti-HB-GAM
Detection system:	horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS14: heart	Strong

Structure	Level	Pattern	Image	Note
TS15: embryo	Present	Widespread		Expression associated with ectodermal structures, mesoderm- and neuroectoderm-derived cells, and their extracellular matrices.
TS15: future brain	Present			Expression localized in neural epithelium from earliest stage analyzed. Expression restricted to some areas of brain and neural tube.
TS15: neural tube	Present			Expression localized in neural epithelium from earliest stage analyzed. Expression restricted to some areas of brain and neural tube.
TS15: spinal ganglion or nerve	Present

Structure	Level	Pattern	Image	Note
TS20: trachea epithelium	Weak
TS20: trachea mesenchyme	Absent

Structure	Level	Pattern	Image	Note
TS21: olfactory epithelium	Present
TS21: nasal mesenchyme	Present	Regionally restricted		Expression in mesenchyme surrounding olfactory epithelium. No expression in respiratory part of olfactory mesenchyme.
TS21: nasal cavity respiratory epithelium	Weak

Structure	Level	Pattern	Image	Note
TS22: heart	Strong		2B
TS22: atrium cardiac muscle	Present		2B	Expression in musculature of ventricle and atrium of the heart.
TS22: ventricle cardiac muscle	Present		2B	Expression in musculature of ventricle and atrium of the heart.
TS22: brain	Present	Widespread	2B	Expression in cerebral hemispheres, telencephalon, diencephalon and corpus striatum
TS22: neocortex	Present	Regionally restricted	2B	Expression in roof of neopallial cortex, in intermediate and ventricular zone. No expression in anuclear layer.
TS22: midbrain roof plate	Strong		2B
TS22: olfactory lobe	Strong		2B
TS22: spinal cord	Present	Diffuse	2B
TS22: face	Strong	Regionally restricted	2B	Strong expression in epithelium and underlying proliferating mesenchyme of facial processes and developing limb.
TS22: limb	Strong	Regionally restricted	2B	Strong expression in epithelium and underlying proliferating mesenchyme of facial processes and developing limb.

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Strong		4A
TS22: neocortex	Strong		4A
TS22: cranium bone primordium	Present		4A

Structure	Level	Pattern	Image	Note
TS22: dorsal root ganglion	Present		4B

Structure	Level	Pattern	Image	Note
TS22: peripheral nerve	Strong		4C	Strong expression in nerve fibers.
TS22: vibrissa follicle	Strong	Regionally restricted	4C	Expression only in ectodermal cells of developing whisker follicles.

Structure	Level	Pattern	Image	Note
TS22: vibrissa follicle	Present	Regionally restricted	4D	Expression only in ectodermal cells of developing whisker follicles.

Structure	Level	Pattern	Image	Note
TS22: peripheral nerve	Present		4E	Expression in neurons innervating whisker follicle.

Structure	Level	Pattern	Image	Note
TS22: spinal cord	Present	Regionally restricted	4F	Authors report intracellular expression. Occasional nuclear expression in cells situated in ventral part of neural tube.

Structure	Level	Pattern	Image	Note
TS22: bronchus epithelium	Weak		4G
TS22: lung parenchyma	Absent		4G

Structure	Level	Pattern	Image	Note
TS22: submandibular gland primordium epithelium	Present	Regionally restricted	4H	Expression localized on cell surfaces of branching epithelial buds.

Structure	Level	Pattern	Image	Note
TS22: vestibular component epithelium	Present		4I	In inner ear, expression in thickened part of vestibular epithelium, in surrounding mesenchyme, and in nerve fibers.
TS22: vestibular component mesenchyme	Present		4I	In inner ear, expression in thickened part of vestibular epithelium, in surrounding mesenchyme, and in nerve fibers.

Structure	Level	Pattern	Image	Note
TS22: striated muscle tissue	Present		5Ab	Expression in differentiating muscles.

Structure	Level	Pattern	Image	Note
TS22: maxilla	Present		5Bb

Structure	Level	Pattern	Image	Note
TS22: meninges	Strong		5Cb
TS22: cranium bone primordium	Present		5Cb

Structure	Level	Pattern	Image	Note
TS22: rib cartilage condensation	Present	Regionally restricted	5Db	No expression in perichondrium.

Structure	Level	Pattern	Image	Note
TS22: liver	Present		5Eb

Structure	Level	Pattern	Image	Note
TS21: bronchus epithelium	Weak		5Fb
TS21: lung parenchyma	Absent		5Fb

Structure	Level	Pattern	Image	Note
TS21: mesenchyme of metanephros	Present		5Gb
TS21: renal tubule	Present		5Gb

Structure	Level	Pattern	Image	Note
TS22: intestine epithelium	Present		5Hb

Structure	Level	Pattern	Image	Note
TS22: adrenal gland	Present		5Ib

Structure	Level	Pattern	Image	Note
TS24: digit skin	Weak	Regionally restricted	5Jb	Weak expression in surface epithelium digits.

Structure	Level	Pattern	Image	Note
TS22: tongue epithelium	Strong
TS22: cartilage	Moderate
TS22: inner ear epithelium	Moderate
TS22: metanephros epithelium	Moderate
TS22: olfactory epithelium	Moderate
TS22: pancreas epithelium	Moderate
TS22: salivary gland epithelium	Moderate
TS22: stomach epithelium	Moderate
TS22: tooth epithelium	Moderate
TS22: tooth mesenchyme	Moderate
TS22: blood vessel endothelium	Weak
TS22: digit mesenchyme	Weak
TS22: esophagus epithelium	Weak
TS22: eye epithelium	Weak
TS22: lung epithelium	Weak
TS22: testis	Weak
TS22: urothelium of ureter	Weak
TS22: stomach mesenchyme	Weak	Regionally restricted		Expression in outer mesenchymal layer which gives rise to smooth muscle layer.
TS22: eye mesenchyme	Ambiguous
TS22: face mesenchyme	Ambiguous
TS22: inner ear mesenchyme	Ambiguous
TS22: lung mesenchyme	Ambiguous
TS22: pancreas mesenchyme	Ambiguous
TS22: salivary gland mesenchyme	Ambiguous
TS22: esophagus mesenchyme	Absent
TS22: intestine mesenchyme	Absent
TS22: mesenchyme of metanephros	Absent
TS22: nasal mesenchyme	Absent
TS22: ureteral mesenchyme	Absent

Structure	Level	Pattern	Image	Note
TS22: medulla oblongata	Present	Regionally restricted		Expression only in some cells.
TS22: choroid plexus	Trace
TS22: sternum	Present	Regionally restricted		Expression in cartilage of developing sternum.
TS22: bone	Present	Regionally restricted		Expression in immature bone matrix. Expression in differentiated osteoblasts and osteocytes. No expression in mature bone.
TS22: palatal rugae epithelium	Present	Regionally restricted		Expression at sites of oral epithelium where palatal rugae are formed.
TS22: genital tubercle epithelium	Present	Regionally restricted		Expression on surfaces of epithelial cells and in peripheral and central core mesenchyme of genital tubercle.
TS22: genital tubercle mesenchyme	Present	Regionally restricted		Expression on surfaces of epithelial cells and in peripheral and central core mesenchyme of genital tubercle.

Structure	Level	Pattern	Image	Note
TS24: embryo	Present	Regionally restricted
TS24: brain	Weak			Authors report expression persisted in a wide variety of tissues.
TS24: peripheral nervous system	Strong			Faint expression in restricted areas of brain.
TS24: heart	Present
TS24: dermis	Present	Single cells		Expression in single cells in the dermis, presumably macrophages.
TS24: cartilage	Present	Regionally restricted		Expression in chondrocytes of several cartilaginous structures.
TS24: bone	Present
TS24: muscle tissue	Present
TS24: inner ear mesenchyme	Present
TS24: vibrissa follicle	Weak	Regionally restricted		Faint expression in epithelium and some mesenchymal cells of whisker follicle.
TS24: genital tubercle	Present