Antibody:	anti-MK
Detection system:	horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS14: heart	Moderate

Structure	Level	Pattern	Image	Note
TS15: embryo	Present	Widespread		Expression associated with ectodermal structures, mesoderm- and neuroectoderm-derived cells, and their extracellular matrices.
TS15: neural ectoderm	Present			Expression localized in neural epithelium from earliest stage analyzed.
TS15: future brain	Present	Widespread
TS15: neural tube	Present	Regionally restricted		Expression in dorsal part of neural tube.
TS15: ganglion	Absent			No expression in ganglia and neuronal projections.

Structure	Level	Pattern	Image	Note
TS20: trachea epithelium	Strong
TS20: trachea mesenchyme	Strong

Structure	Level	Pattern	Image	Note
TS21: olfactory epithelium	Present
TS21: nasal mesenchyme	Present	Regionally restricted		Expression in mesenchyme surrounding olfactory epithelium. Authors report no expression in respiratory part of mesenchyme.
TS21: nasal cavity respiratory epithelium	Weak

Structure	Level	Pattern	Image	Note
TS22: brain	Present	Widespread	2A	Authors report expression in cerebral hemispheres, telencephalon, diencephalon and corpus striatum.
TS22: spinal cord	Present	Regionally restricted	2A	In neural tube, expression restricted to one part of dorsal area.
TS22: heart valve	Present		2A	In the heart, expression only in the valves.
TS22: olfactory epithelium	Present	Regionally restricted	2A	Restricted expression in only parts of olfactory epithelium.
TS22: lung	Present	Regionally restricted	2A	Expression distributed on surfaces of epithelial cells of intrapulmonary segmental bronchi, bronchioli, alveolar ducts and surrounding mesenchyme.
TS22: intestine	Present	Regionally restricted	2A	In intestine, expression in both mucosal epithelium and mesnchyme.
TS22: facial bone primordium	Strong	Regionally restricted	2A	Strong expression in epithelium and underlying proliferating mesenchyme of facial processes and developing limb.
TS22: limb digit	Strong	Regionally restricted	2A	Strong expression in epithelium and underlying proliferating mesenchyme of facial processes and developing limb.

Structure	Level	Pattern	Image	Note
TS21: gut	Weak		3A
TS21: liver	Weak		3A
TS21: pancreas	Weak		3A
TS21: testis	Weak		3A
TS21: mesenchyme of metanephros	Present		3A
TS21: renal tubule	Present		3A

Structure	Level	Pattern	Image	Note
TS22: neocortex	Strong		3B
TS22: choroid plexus	Absent		3B

Structure	Level	Pattern	Image	Note
TS21: rib cartilage condensation	Present		3C

Structure	Level	Pattern	Image	Note
TS21: mandible	Strong	Regionally restricted	3D
TS21: tooth bud	Strong		3D
TS21: tongue epithelium	Strong		3D

Structure	Level	Pattern	Image	Note
TS21: Meckel's cartilage	Strong	Regionally restricted	3E	Strong expression in perichondrium and hypertrophied chondrocytes.
TS21: lower jaw skeleton	Strong	Regionally restricted	3E	Strong expression in immature bone matrix. No expression in mature bone.

Structure	Level	Pattern	Image	Note
TS22: vestibular component epithelium	Present	Regionally restricted	3F	Expression in thickened part of vestibular epithelium.
TS22: inner ear mesenchyme	Present	Regionally restricted	3F	Expression in mesenchyme surrounding vestibular epithelium and in nerve fibers.
TS22: otic capsule	Present	Regionally restricted	3F	Expression in cartilaginous otic capsule.

Structure	Level	Pattern	Image	Note
TS22: stomach epithelium	Strong	Regionally restricted	3G
TS22: stomach mesenchyme	Strong	Regionally restricted	3G

Structure	Level	Pattern	Image	Note
TS22: submandibular gland primordium	Present	Regionally restricted	3H	Expression localized on cell surfaces of branchial epithelial buds, in basement membranes and in mesenchyme.

Structure	Level	Pattern	Image	Note
TS21: olfactory epithelium	Present	Regionally restricted	3I	Expression in nerve fibers.

Structure	Level	Pattern	Image	Note
TS22: mandible	Strong	Regionally restricted	3J	Strong expression in underlying mesenchyme and basement membrane.

Structure	Level	Pattern	Image	Note
TS24: dermis	Present	Single cells	3K	Expression in single cells in the dermis, presumably macrophages.

Structure	Level	Pattern	Image	Note
TS22: striated muscle tissue	Present		5Aa	Expression in differentiating muscles.

Structure	Level	Pattern	Image	Note
TS22: maxilla	Present	Regionally restricted	5Ba	Expression in differentiating osteoblast and osteocytes.

Structure	Level	Pattern	Image	Note
TS22: meninges	Strong		5Ca
TS22: cranium bone primordium	Present		5Ca

Structure	Level	Pattern	Image	Note
TS22: rib cartilage condensation	Strong	Regionally restricted	5Da	Strong expression in perichondrium and hypertrophied chondrocytes.

Structure	Level	Pattern	Image	Note
TS22: liver	Weak		5Ea

Structure	Level	Pattern	Image	Note
TS21: lung epithelium	Present	Regionally restricted	5Fa	Expression distributed on surfaces of epithelial cells of intrapulmonary segmental bronchi, bronchioli, alveolar ducts and surrounding mesenchyme.

Structure	Level	Pattern	Image	Note
TS21: mesenchyme of metanephros	Present		5Ga
TS21: renal tubule	Present		5Ga

Structure	Level	Pattern	Image	Note
TS22: intestine epithelium	Present		5Ha
TS22: intestine mesenchyme	Present		5Ha

Structure	Level	Pattern	Image	Note
TS22: adrenal gland	Weak		5Ia

Structure	Level	Pattern	Image	Note
TS24: digit mesenchyme	Strong	Regionally restricted	5Ja

Structure	Level	Pattern	Image	Note
TS21: limb epithelium	Strong		7Aa
TS21: limb mesenchyme	Strong		7Aa

Structure	Level	Pattern	Image	Note
TS22: submandibular gland primordium epithelium	Present	Regionally restricted	7Ba	Expression localized on cell surfaces of branchial epithelial buds, in basement membranes and in mesenchyme.
TS22: submandibular gland primordium mesenchyme	Present	Regionally restricted	7Ba	Expression localized on cell surfaces of branchial epithelial buds, in basement membranes and in mesenchyme.

Structure	Level	Pattern	Image	Note
TS21: vibrissa follicle	Present	Regionally restricted	7Ca	Expression in both components of whisker follicles.

Structure	Level	Pattern	Image	Note
TS22: hair follicle	Strong	Regionally restricted	7Da	Intense expression on surfaces of both epidermal and mesenchymal cells at sites of hair and whisker follicles.
TS22: blood vessel endothelium	Weak	Regionally restricted	7Da	Weak expression in endothelial cells and basement membranes of blood vessels.

Structure	Level	Pattern	Image	Note
TS21: dorsal root ganglion	Present		7Ea

Structure	Level	Pattern	Image	Note
TS22: palatal rugae epithelium	Present		7Fa	Expression at sites of oral epithelium where palatal rugae are formed.
TS22: tongue epithelium	Present		7Fa

Structure	Level	Pattern	Image	Note
TS22: vibrissa follicle	Strong	Regionally restricted		Intense expression in both components of whisker \
TS22: cartilage	Strong
TS22: digit mesenchyme	Strong
TS22: eye epithelium	Strong
TS22: face mesenchyme	Strong
TS22: inner ear epithelium	Strong
TS22: intestine epithelium	Strong
TS22: lung epithelium	Strong
TS22: metanephros epithelium	Strong
TS22: olfactory epithelium	Strong
TS22: salivary gland epithelium	Strong
TS22: tongue epithelium	Strong
TS22: tooth epithelium	Strong
TS22: tooth mesenchyme	Strong
TS22: dermis	Strong	Regionally restricted		Strong expression in dermis epithelium.v
TS22: eye mesenchyme	Moderate
TS22: intestine mesenchyme	Moderate
TS22: pancreas epithelium	Moderate
TS22: urothelium of ureter	Moderate
TS22: stomach	Present	Regionally restricted		Expression in stomach musculature.v
TS22: esophagus epithelium	Weak
TS22: inner ear mesenchyme	Weak
TS22: lung mesenchyme	Weak
TS22: mesenchyme of metanephros	Weak
TS22: nasal mesenchyme	Weak
TS22: oral epithelium	Weak
TS22: pancreas mesenchyme	Weak
TS22: salivary gland mesenchyme	Weak
TS22: tail nerve	Weak
TS22: testis	Weak
TS22: ureteral mesenchyme	Weak
TS22: blood vessel endothelium	Ambiguous
TS22: heart	Ambiguous
TS22: spinal cord	Ambiguous
TS22: choroid plexus	Absent
TS22: dorsal root ganglion	Absent
TS22: esophagus mesenchyme	Absent

Structure	Level	Pattern	Image	Note
TS22: neocortex	Strong	Regionally restricted		Expression in roof of neopallial cortex, in intermediate and ventricular zone. Expression also in anuclear layer.
TS22: midbrain roof plate	Strong
TS22: olfactory lobe	Strong
TS22: medulla oblongata	Present	Regionally restricted		Expression only in some cells.
TS22: lens	Present
TS22: neural retina	Present
TS22: perioptic mesenchyme	Present
TS22: nasal capsule	Present	Regionally restricted		Expression in precartilaginous mesenchymal condensations of otic and nose capsule.
TS22: otic capsule	Present	Regionally restricted		Expression in precartilaginous mesenchymal condensations of otic and nose capsule.
TS22: sternebra pre-cartilage condensation	Absent			No expression in cartilage of developing sternum.
TS22: genital tubercle epithelium	Present
TS22: genital tubercle mesenchyme	Present	Regionally restricted		Expression in peripheral and central core mesenchyme of genital tubercle.

Structure	Level	Pattern	Image	Note
TS24: brain	Weak	Regionally restricted		Faint expression in restricted areas of brain.
TS24: heart	Absent
TS24: cartilage	Present	Regionally restricted		Expression in chondrocytes of several cartilaginous structures.
TS24: bone	Absent
TS24: muscle tissue	Absent
TS24: inner ear epithelium	Trace	Regionally restricted		Very faint expression in sensory epithelium of inner ear.
TS24: olfactory epithelium	Weak			Reduced levels of expression in olfactory epithelium.
TS24: vibrissa follicle	Weak	Regionally restricted		Weak expression only in mesenchymal component of whisker follicle.
TS24: esophagus	Present
TS24: duodenum epithelium	Present			Expression in duodenum distributed in both epithelium and mesenchyme.
TS24: duodenum mesenchyme	Present			Expression in duodenum distributed in both epithelium and mesenchyme.
TS24: midgut	Present	Regionally restricted		Expression in midgut mesenchymal cells surrounding the mucosa.
TS24: genital tubercle	Absent			No expression in genital organ.
TS24: face	Absent			No expression in facial processes.