Gene Expression Data
Assay Details
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Assay
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Reference:
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J:157265
McKnight KD, et al., Foxh1 and Foxa2 are not required for formation of the midgut and hindgut definitive endoderm. Dev Biol. 2010 Jan 15;337(2):471-81
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6258938
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Gene symbol:
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Trh
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Gene name:
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thyrotropin releasing hormone
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Probe:
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Trh cDNA
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
2A:
embryonic day 8.5
(more  )
Specimen
2A:
(close  )
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Genetic Background:
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involves: 129 * ICR
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2B:
embryonic day 8.5
(more )
Specimen
2B:
(close )
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Genetic Background:
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involves: 129 * ICR
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2C:
embryonic day 8.5
(more )
Specimen
2C:
(close )
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Genetic Background:
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involves: 129 * ICR
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
2C inset:
embryonic day 8.5
(more )
Specimen
2C inset:
(close )
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Genetic Background:
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involves: 129 * ICR
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
2D:
embryonic day 8.5
(more )
Specimen
2D:
(close )
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Genetic Background:
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involves: 129 * ICR
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
2E:
(close )
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Genetic Background:
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involves: 129 * ICR
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Mutant Allele(s):
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Foxa2tm1Dnl/Foxa2tm1Dnl
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2F:
(close )
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Genetic Background:
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involves: 129 * ICR
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Mutant Allele(s):
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Foxa2tm1Dnl/Foxa2tm1Dnl
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2G:
(close )
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Genetic Background:
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involves: 129 * ICR
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Mutant Allele(s):
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Foxa2tm1Dnl/Foxa2tm1Dnl
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: foregut diverticulum endoderm |
Present |
Regionally restricted |
2G
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Expression across midline. Authors state that notochordal plate cells are absent.
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Specimen
2H:
(close )
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Genetic Background:
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involves: 129 * ICR
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Mutant Allele(s):
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Foxa2tm1Dnl/Foxa2tm1Dnl
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
2I:
(close )
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Genetic Background:
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involves: 129 * ICR
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Mutant Allele(s):
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Foxh1tm1Jlw/Foxh1tm1Jlw
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2J:
(close )
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Genetic Background:
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involves: 129 * ICR
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Mutant Allele(s):
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Foxh1tm1Jlw/Foxh1tm1Jlw
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2K:
(close )
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Genetic Background:
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involves: 129 * ICR
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Mutant Allele(s):
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Foxh1tm1Jlw/Foxh1tm1Jlw
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
2K1:
(close )
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Genetic Background:
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involves: 129 * ICR
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Mutant Allele(s):
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Foxh1tm1Jlw/Foxh1tm1Jlw
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
2K2:
(close )
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Genetic Background:
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involves: 129 * ICR
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Mutant Allele(s):
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Foxh1tm1Jlw/Foxh1tm1Jlw
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3G:
embryonic day 7.5
(more )
Specimen
3G:
(close )
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Genetic Background:
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involves: 129 * ICR
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: definitive endoderm |
Present |
Widespread |
3G
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Expression throughout the definitive endoderm, with higher expression in the anterior region.
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Specimen
3H:
(close )
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Genetic Background:
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involves: 129 * ICR
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Mutant Allele(s):
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Foxa2tm1Dnl/Foxa2tm1Dnl
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: definitive endoderm |
Weak |
Regionally restricted |
3H
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Significantly reduced levels of expression in anterior-distal and posterior-distal regions.
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Specimen
3I:
(close )
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Genetic Background:
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involves: 129 * ICR
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Mutant Allele(s):
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Foxh1tm1Jlw/Foxh1tm1Jlw
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: definitive endoderm |
Present |
Regionally restricted |
3I
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Expression in anterior-distal region of embryo.
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