Probe:	Enc1 probe1
Probe preparation:	labelled with digoxigenin
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS19: midbrain	Present		7A E11.5 Lateral	Expression throughout midbrain area to prosomere 3 area from E11.5 to E14.5
TS19: prosomere 1	Present		7A E11.5 Lateral	Expression throughout midbrain area to prosomere 3 area from E11.5 to E14.5
TS19: prosomere 2	Present		7A E11.5 Lateral	Expression throughout midbrain area to prosomere 3 area from E11.5 to E14.5
TS19: prosomere 3	Present		7A E11.5 Lateral	Expression throughout midbrain area to prosomere 3 area from E11.5 to E14.5

Structure	Level	Pattern	Image	Note
TS19: midbrain	Present		7A E11.5 Medial	Expression throughout midbrain area to prosomere 3 area from E11.5 to E14.5
TS19: prosomere 1	Present		7A E11.5 Medial	Expression throughout midbrain area to prosomere 3 area from E11.5 to E14.5
TS19: prosomere 2	Present		7A E11.5 Medial	Expression throughout midbrain area to prosomere 3 area from E11.5 to E14.5
TS19: prosomere 3	Present		7A E11.5 Medial	Expression throughout midbrain area to prosomere 3 area from E11.5 to E14.5

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present		7A E12.5 Lateral	Expression throughout midbrain area to prosomere 3 area from E11.5 to E14.5
TS20: prosomere 1	Present		7A E12.5 Lateral	Expression throughout midbrain area to prosomere 3 area from E11.5 to E14.5
TS20: prosomere 2	Present		7A E12.5 Lateral	Expression throughout midbrain area to prosomere 3 area from E11.5 to E14.5
TS20: prosomere 3	Present		7A E12.5 Lateral	Expression throughout midbrain area to prosomere 3 area from E11.5 to E14.5

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present		7A E12.5 Medial	Expression throughout midbrain area to prosomere 3 area from E11.5 to E14.5
TS20: prosomere 1	Present		7A E12.5 Medial	Expression throughout midbrain area to prosomere 3 area from E11.5 to E14.5
TS20: prosomere 2	Present		7A E12.5 Medial	Expression throughout midbrain area to prosomere 3 area from E11.5 to E14.5
TS20: prosomere 3	Present		7A E12.5 Medial	Expression throughout midbrain area to prosomere 3 area from E11.5 to E14.5

Structure	Level	Pattern	Image	Note
TS20: prosomere 2	Present	Regionally restricted	7A1	Specific expression overlaps with rostrally located group of Th-expressing neurons in prosomere 2-3 area.
TS20: prosomere 3	Present	Regionally restricted	7A1	Specific expression overlaps with rostrally located group of Th-expressing neurons in prosomere 2-3 area.

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present		7A E14.5 Lateral	Expression throughout midbrain area to prosomere 3 area from E11.5 to E14.5
TS22: prosomere 1	Present		7A E14.5 Lateral	Expression throughout midbrain area to prosomere 3 area from E11.5 to E14.5
TS22: prosomere 2	Present		7A E14.5 Lateral	Expression throughout midbrain area to prosomere 3 area from E11.5 to E14.5
TS22: prosomere 3	Present		7A E14.5 Lateral	Expression throughout midbrain area to prosomere 3 area from E11.5 to E14.5

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present		7A E14.5 Medial	Expression throughout midbrain area to prosomere 3 area from E11.5 to E14.5
TS22: prosomere 1	Present		7A E14.5 Medial	Expression throughout midbrain area to prosomere 3 area from E11.5 to E14.5
TS22: prosomere 2	Present		7A E14.5 Medial	Expression throughout midbrain area to prosomere 3 area from E11.5 to E14.5
TS22: prosomere 3	Present		7A E14.5 Medial	Expression throughout midbrain area to prosomere 3 area from E11.5 to E14.5

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present	Regionally restricted	7A2	Expression overlaps with rostrally located group of Th-expressing neurons.

Structure	Level	Pattern	Image	Note
TS19: midbrain floor plate	Present	Regionally restricted	7B E11.5 Rostral	Specific expression in subventricular zone of floor plate and basal plate of midbrain.

Structure	Level	Pattern	Image	Note
TS19: midbrain mantle layer	Present	Regionally restricted	7B E11.5 Caudal	Specific expression in subventricular zone of floor plate and basal plate of midbrain.

Structure	Level	Pattern	Image	Note
TS20: midbrain floor plate	Present	Regionally restricted	7B E12.5 Rostral	Specific expression in subventricular zone of floor plate and basal plate of midbrain.

Structure	Level	Pattern	Image	Note
TS20: midbrain mantle layer	Present	Regionally restricted	7B E12.5 Caudal	Specific expression in subventricular zone of floor plate and basal plate of midbrain.

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present	Regionally restricted	7B1	Expression overlaps with rostrally located Th-expressing neurons.

Structure	Level	Pattern	Image	Note
TS22: midbrain floor plate	Present	Regionally restricted	7B E14.5 Rostral	Specific expression in subventricular zone of floor plate and basal plate of midbrain.

Structure	Level	Pattern	Image	Note
TS22: midbrain mantle layer	Present	Regionally restricted	7B E14.5 Caudal	Specific expression in subventricular zone of floor plate and basal plate of midbrain.

Structure	Level	Pattern	Image	Note
TS22: midbrain mantle layer	Present	Regionally restricted	7B2	Expression in some Th-expressing neurons in subventricular zone.

Structure	Level	Pattern	Image	Note
TS28: substantia nigra	Weak	Regionally restricted	7B Adult	Expression in part of Th-expressing neurons of substantia nigra.

Structure	Level	Pattern	Image	Note
TS28: substantia nigra	Weak	Regionally restricted	7B3	Expression in part of Th-expressing neurons of substantia nigra.