About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:260421 Mesman S, et al., Expression analyzes of early factors in midbrain differentiation programs. Gene Expr Patterns. 2018 Jan;27:8-15
Assay type: RNA in situ
MGI Accession ID: MGI:6258332
Gene symbol: Tcf3
Gene name: transcription factor 3
Probe: Tcf3 probe2
Probe preparation: labelled with digoxigenin
Visualized with: Alkaline phosphatase
Results
Specimen 6A E11.5 Lateral: embryonic day 11.5 (more )
Note: Double labeled: blue - Tcf3; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS19: hindbrain Present 6A E11.5 Lateral Expression from hindbrain, throughout midbrain into prosomere 3 areas.
TS19: midbrain Present 6A E11.5 Lateral Expression from hindbrain, throughout midbrain into prosomere 3 areas.
TS19: prosomere 1 Present 6A E11.5 Lateral Expression from hindbrain, throughout midbrain into prosomere 3 areas.
TS19: prosomere 2 Present 6A E11.5 Lateral Expression from hindbrain, throughout midbrain into prosomere 3 areas.
TS19: prosomere 3 Present 6A E11.5 Lateral Expression from hindbrain, throughout midbrain into prosomere 3 areas.
Specimen 6A E11.5 Medial: embryonic day 11.5 (more )
Note: Double labeled: blue - Tcf3; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS19: midbrain Ambiguous 6A E11.5 Medial
Specimen 6A E12.5 Lateral: embryonic day 12.5 (more )
Note: Double labeled: blue - Tcf3; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS20: hindbrain Present 6A E12.5 Lateral Expression from hindbrain, throughout midbrain into prosomere 3 areas.
TS20: midbrain Present 6A E12.5 Lateral Expression from hindbrain, throughout midbrain into prosomere 3 areas.
TS20: prosomere 1 Present 6A E12.5 Lateral Expression from hindbrain, throughout midbrain into prosomere 3 areas.
TS20: prosomere 2 Present 6A E12.5 Lateral Expression from hindbrain, throughout midbrain into prosomere 3 areas.
TS20: prosomere 3 Present 6A E12.5 Lateral Expression from hindbrain, throughout midbrain into prosomere 3 areas.
Specimen 6A E12.5 Medial: embryonic day 12.5 (more )
Note: Double labeled: blue - Tcf3; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS20: midbrain Present Regionally restricted 6A E12.5 Medial Expression overlaps with Th-expressing neuronal popolation in medial part of midbrain.
Specimen 6A1: embryonic day 12.5 (more )
Note: Double labeled: blue - Tcf3; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS20: midbrain Present Regionally restricted 6A1 Expression overlaps with Th-expressing neuronal popolation in medial part of midbrain.
Specimen 6A E14.5 Lateral: embryonic day 14.5 (more )
Note: Double labeled: blue - Tcf3; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS22: midbrain Ambiguous 6A E14.5 Lateral
Specimen 6A E14.5 Medial: embryonic day 14.5 (more )
Note: Double labeled: blue - Tcf3; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS22: midbrain Present Regionally restricted 6A E14.5 Medial Exprexsion overlaps with Th-expressing neuronal popolation in medial part of midbrain.
Specimen 6A2: embryonic day 14.5 (more )
Note: Double labeled: blue - Tcf3; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS22: midbrain Present Regionally restricted 6A2 Exprexsion overlaps with Th-expressing neuronal popolation in medial part of midbrain.
Specimen 6B E11.5 Rostral: embryonic day 11.5 (more )
Note: Double labeled: blue - Tcf3; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS19: midbrain floor plate Present Regionally restricted 6B E11.5 Rostral Expression specific to ventricular zone of floor plate and basal plate of midbrain.
Specimen 6B E11.5 Caudal: embryonic day 11.5 (more )
Note: Double labeled: blue - Tcf3; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS19: midbrain ventricular layer Present Regionally restricted 6B E11.5 Caudal Expression specific to ventricular zone of floor plate and basal plate of midbrain.
Specimen 6B E12.5 Rostral: embryonic day 12.5 (more )
Note: Double labeled: blue - Tcf3; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS20: midbrain floor plate Present Regionally restricted 6B E12.5 Rostral Expression specific to ventricular zone of floor plate and basal plate of midbrain.
Specimen 6B E12.5 Caudal: embryonic day 12.5 (more )
Note: Double labeled: blue - Tcf3; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS20: midbrain ventricular layer Present Regionally restricted 6B E12.5 Caudal Expression specific to ventricular zone of floor plate and basal plate of midbrain.
Specimen 6B1: embryonic day 12.5 (more )
Note: Double labeled: blue - Tcf3; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS20: midbrain Present Regionally restricted 6B1 Expression specific to ventricular zone of floor plate and basal plate of midbrain.
Specimen 6B E14.5 Rostral: embryonic day 14.5 (more )
Note: Double labeled: blue - Tcf3; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS22: midbrain floor plate Present Regionally restricted 6B E14.5 Rostral Expression specific to ventricular zone of floor plate and basal plate of midbrain.
Specimen 6B E14.5 Caudal: embryonic day 14.5 (more )
Note: Double labeled: blue - Tcf3; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS22: midbrain ventricular layer Present Regionally restricted 6B E14.5 Caudal Expression specific to ventricular zone of floor plate and basal plate of midbrain.
Specimen 6B2: embryonic day 14.5 (more )
Note: Double labeled: blue - Tcf3; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS22: midbrain Present Regionally restricted 6B2 Expression overlaps with medially located Th-expressing neurons.
Specimen 6B Adult: postnatal adult (more )
Note: Double labeled: blue - Tcf3; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS28: midbrain Absent 6B Adult No expression in adult mdDA (mesodiencephalic dopaminergic) neuronal population.
Specimen 6B3: postnatal adult (more )
Note: Double labeled: blue - Tcf3; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS28: midbrain Absent 6B3 No expression in adult mdDA (mesodiencephalic dopaminergic) neuronal population.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory