Gene Expression Data
Assay Details
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Assay
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Reference:
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J:260421
Mesman S, et al., Expression analyzes of early factors in midbrain differentiation programs. Gene Expr Patterns. 2018 Jan;27:8-15
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6258329
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Gene symbol:
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Tcf12
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Gene name:
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transcription factor 12
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Probe:
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Tcf12 probe1
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Probe preparation:
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labelled with digoxigenin
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
4A E11.5 Lateral:
embryonic day 11.5
(more  )
Note: Double labeled: blue - Tcf12; brown - Th (assay MGI:6258336).
Specimen
4A E11.5 Lateral:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Tcf12; brown - Th (assay MGI:6258336).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: midbrain |
Present |
Regionally restricted |
4A E11.5 Lateral
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From E11.5 onwards, expression in medial midbrain area from isthmus organizer up to the prosomere 3 area.
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| TS19: midbrain-hindbrain junction |
Present |
Regionally restricted |
4A E11.5 Lateral
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From E11.5 onwards, expression in medial midbrain area from isthmus organizer up to the prosomere 3 area.
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| TS19: prosomere 1 |
Present |
Regionally restricted |
4A E11.5 Lateral
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From E11.5 onwards, expression in medial midbrain area from isthmus organizer up to the prosomere 3 area.
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| TS19: prosomere 2 |
Present |
Regionally restricted |
4A E11.5 Lateral
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From E11.5 onwards, expression in medial midbrain area from isthmus organizer up to the prosomere 3 area.
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| TS19: prosomere 3 |
Present |
Regionally restricted |
4A E11.5 Lateral
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From E11.5 onwards, expression in medial midbrain area from isthmus organizer up to the prosomere 3 area.
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Specimen
4A E11.5 Medial:
embryonic day 11.5
(more )
Note: Double labeled: blue - Tcf12; brown - Th (assay MGI:6258336).
Specimen
4A E11.5 Medial:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Cryosection
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|
Note:
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Double labeled: blue - Tcf12; brown - Th (assay MGI:6258336).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: midbrain |
Present |
Regionally restricted |
4A E11.5 Medial
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From E11.5 onwards, expression in medial midbrain area from isthmus organizer up to the prosomere 3 area.
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| TS19: midbrain-hindbrain junction |
Present |
Regionally restricted |
4A E11.5 Medial
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From E11.5 onwards, expression in medial midbrain area from isthmus organizer up to the prosomere 3 area.
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| TS19: prosomere 1 |
Present |
Regionally restricted |
4A E11.5 Medial
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From E11.5 onwards, expression in medial midbrain area from isthmus organizer up to the prosomere 3 area.
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| TS19: prosomere 2 |
Present |
Regionally restricted |
4A E11.5 Medial
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From E11.5 onwards, expression in medial midbrain area from isthmus organizer up to the prosomere 3 area.
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| TS19: prosomere 3 |
Present |
Regionally restricted |
4A E11.5 Medial
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From E11.5 onwards, expression in medial midbrain area from isthmus organizer up to the prosomere 3 area.
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Specimen
4A E12.5 Lateral:
embryonic day 12.5
(more )
Note: Double labeled: blue - Tcf12; brown - Th (assay MGI:6258336).
Specimen
4A E12.5 Lateral:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Tcf12; brown - Th (assay MGI:6258336).
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Specimen
4A E12.5 Medial:
embryonic day 12.5
(more )
Note: Double labeled: blue - Tcf12; brown - Th (assay MGI:6258336).
Specimen
4A E12.5 Medial:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: blue - Tcf12; brown - Th (assay MGI:6258336).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: midbrain |
Present |
Regionally restricted |
4A E12.5 Medial
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Expression overlaps with medially localized Th-expressing neurons.
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Specimen
4A1:
embryonic day 12.5
(more )
Note: Double labeled: blue - Tcf12; brown - Th (assay MGI:6258336).
Specimen
4A1:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: blue - Tcf12; brown - Th (assay MGI:6258336).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: midbrain |
Present |
Regionally restricted |
4A1
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Expression overlaps with medially localized Th-expressing neurons.
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Specimen
4A E14.5 Lateral:
embryonic day 14.5
(more )
Note: Double labeled: blue - Tcf12; brown - Th (assay MGI:6258336).
Specimen
4A E14.5 Lateral:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: blue - Tcf12; brown - Th (assay MGI:6258336).
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Specimen
4A E14.5 Medial:
embryonic day 14.5
(more )
Note: Double labeled: blue - Tcf12; brown - Th (assay MGI:6258336).
Specimen
4A E14.5 Medial:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Cryosection
|
|
Note:
|
Double labeled: blue - Tcf12; brown - Th (assay MGI:6258336).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
Present |
Regionally restricted |
4A E14.5 Medial
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Expression extensively overlaps with medially localized Th-expressing neurons.
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Specimen
4A2:
embryonic day 14.5
(more )
Note: Double labeled: blue - Tcf12; brown - Th (assay MGI:6258336).
Specimen
4A2:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: blue - Tcf12; brown - Th (assay MGI:6258336).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
Present |
Regionally restricted |
4A2
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Expression extensively overlaps with medially localized Th-expressing neurons.
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Specimen
4B E11.5 Rostral:
embryonic day 11.5
(more )
Note: Double labeled: blue - Tcf12; brown - Th (assay MGI:6258336).
Specimen
4B E11.5 Rostral:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: blue - Tcf12; brown - Th (assay MGI:6258336).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: midbrain floor plate |
Present |
Regionally restricted |
4B E11.5 Rostral
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Expression in ventricular zone and subventricular zone of the entire floor plate region of the midbrain throughout development.
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Specimen
4B E11.5 Caudal:
embryonic day 11.5
(more )
Note: Double labeled: blue - Tcf12; brown - Th (assay MGI:6258336).
Specimen
4B E11.5 Caudal:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: blue - Tcf12; brown - Th (assay MGI:6258336).
|
Specimen
4B E12.5 Rostral:
embryonic day 12.5
(more )
Note: Double labeled: blue - Tcf12; brown - Th (assay MGI:6258336).
Specimen
4B E12.5 Rostral:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: blue - Tcf12; brown - Th (assay MGI:6258336).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: midbrain floor plate |
Present |
Regionally restricted |
4B E12.5 Rostral
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Expression in ventricular zone and subventricular zone of the entire floor plate region of the midbrain throughout development.
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Specimen
4B E12.5 Caudal:
embryonic day 12.5
(more )
Note: Double labeled: blue - Tcf12; brown - Th (assay MGI:6258336).
Specimen
4B E12.5 Caudal:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: blue - Tcf12; brown - Th (assay MGI:6258336).
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Specimen
4B1:
embryonic day 12.5
(more )
Note: Double labeled: blue - Tcf12; brown - Th (assay MGI:6258336).
Specimen
4B1:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: blue - Tcf12; brown - Th (assay MGI:6258336).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: midbrain |
Present |
Regionally restricted |
4B1
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Expression overlaps with Th-expressing neuronal population in medial part of midbrain, most prominent in caudal regions.
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Specimen
4B E14.5 Rostral:
embryonic day 14.5
(more )
Note: Double labeled: blue - Tcf12; brown - Th (assay MGI:6258336).
Specimen
4B E14.5 Rostral:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: blue - Tcf12; brown - Th (assay MGI:6258336).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
Present |
Regionally restricted |
4B E14.5 Rostral
|
Expression overlaps with Th-expressing neuronal population in medial part of midbrain.
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Specimen
4B E14.5 Caudal:
embryonic day 14.5
(more )
Note: Double labeled: blue - Tcf12; brown - Th (assay MGI:6258336).
Specimen
4B E14.5 Caudal:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: blue - Tcf12; brown - Th (assay MGI:6258336).
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Specimen
4B2:
embryonic day 14.5
(more )
Note: Double labeled: blue - Tcf12; brown - Th (assay MGI:6258336).
Specimen
4B2:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
|
section
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|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: blue - Tcf12; brown - Th (assay MGI:6258336).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: midbrain floor plate |
Present |
Regionally restricted |
4B2
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Expression overlaps with Th-expressing neuronal population in medial part of midbrain.
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Specimen
4B Adult:
postnatal adult
(more )
Note: Double labeled: blue - Tcf12; brown - Th (assay MGI:6258336).
Specimen
4B Adult:
(close )
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Genetic Background:
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C57BL/6
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
|
Cryosection
|
|
Note:
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Double labeled: blue - Tcf12; brown - Th (assay MGI:6258336).
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Specimen
4B3:
postnatal adult
(more )
Note: Double labeled: blue - Tcf12; brown - Th (assay MGI:6258336).
Specimen
4B3:
(close )
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Genetic Background:
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C57BL/6
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: blue - Tcf12; brown - Th (assay MGI:6258336).
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