Gene Expression Data
Assay Details
Assay
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Reference:
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J:240035
Roux M, et al., Hoxa1 and Hoxb1 are required for pharyngeal arch artery development. Mech Dev. 2017 Feb;143:1-8
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6257899
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Gene symbol:
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Crabp1
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Gene name:
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cellular retinoic acid binding protein I
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Probe:
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Crabp1 probe14
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
6A:
embryonic day 9.5; Hoxa1tm1Ipc/Hoxa1 +
(more )
Specimen
6A:
(close )
Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Hoxa1tm1Ipc/Hoxa1+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS15: migrating neural crest |
Present |
Regionally restricted |
6A
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Authors report normal migration of neural crest cells to 3rd and 4th pharyngeal arch arteries.
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Specimen
6B:
embryonic day 9.5; Hoxb1tm5Mrc/Hoxb1 +
(more )
Specimen
6B:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hoxb1tm5Mrc/Hoxb1+
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Age:
|
embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: migrating neural crest |
Present |
Regionally restricted |
6B
|
Authors report normal migration of neural crest cells to 3rd and 4th pharyngeal arch arteries.
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Specimen
6C:
(close )
Genetic Background:
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involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hoxa1tm1Ipc/Hoxa1+ Hoxb1tm5Mrc/Hoxb1+
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Age:
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embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: migrating neural crest |
Present |
Regionally restricted |
6C
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Authors report abnormal post-otic streams of migrating neural crest cells.
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Specimen
6D:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hoxb1tm5Mrc/Hoxb1tm5Mrc
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Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: migrating neural crest |
Present |
Regionally restricted |
6D
|
Authors report normal migration of neural crest cells to 3rd and 4th pharyngeal arch arteries.
|
Specimen
6E:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hoxa1tm1Ipc/Hoxa1+ Hoxb1tm5Mrc/Hoxb1tm5Mrc
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Age:
|
embryonic day 9.5
|
Sex:
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Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: migrating neural crest |
Present |
Regionally restricted |
6E
|
Authors report abnormal post-otic streams of migrating neural crest cells.
|
Specimen
6F:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hoxa1tm1Ipc/Hoxa1tm1Ipc Hoxb1tm5Mrc/Hoxb1tm5Mrc
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Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: migrating neural crest |
Present |
Regionally restricted |
6F
|
Authors report normal migration of neural crest cells to 3rd and 4th pharyngeal arch arteries.
|
TS15: 3rd arch artery |
Weak |
Regionally restricted |
6F
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Reduced expression in 3rd pharyngeal arch artery compared to wild type.
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Specimen
S1A:
embryonic day 9.5
(more )
Specimen
S1A:
(close )
Genetic Background:
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involves: C57BL/6
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Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
S1B:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
|
Hoxa1tm1Ipc/Hoxa1tm1Ipc
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: 3rd arch artery |
Weak |
Regionally restricted |
S1B inset
,
S1B
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Reduced expression in 3rd pharyngeal arch artery compared to wild type.
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Specimen
S1C:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hoxa1tm1Ipc/Hoxa1tm1Ipc Hoxb1tm5Mrc/Hoxb1+
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Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: 3rd arch artery |
Weak |
Regionally restricted |
S1C
,
S1C inset
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Reduced expression in 3rd pharyngeal arch artery compared to wild type.
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|