Summary | Mutation origin | Mutation description | Find Mice (IMSR) | Notes | References

Symbol:	Col1a1tm1(tetO-GFP/RNAi:Stat3)Ern
Name:	collagen, type I, alpha 1; targeted mutation 1, Matthias Ernst
MGI ID:	MGI:6257037
Synonyms:	TRE-Stat3.1348
Gene:	Col1a1  Location: Chr11:94827050-94843868 bp, + strand  Genetic Position: Chr11, 59.01 cM, cytoband D
Alliance:	Col1a1tm1(tetO-GFP/RNAi:Stat3)Ern page

Germline Transmission:	Earliest citation of germline transmission: J:239149
Parent Cell Line:	KH2 (ES Cell)
Strain of Origin:	(C57BL/6 x 129S4/SvJae)F1

Allele Type:    Targeted (Inducible, Knockdown, RMCE-ready, Reporter) Inducer:    doxycycline/tetracycline Mutation:    Insertion   Mutation details: RMCE (recombinase-mediated cassette exchange) was utilized to insert tetO-GFP/RNAi:Stat3 into the Col1a1 locus. KH2 embryonic stem cells (C57BL/6 x 129S4Sv/Jae), carrying the Gt(ROSA)26Sortm1(rtTA*M2)Jae allele, were previously re-targeted to insert an FRT site-flanked PGK-Neo cassette and a promoterless hygromycin resistance cassette in the 3' UTR of the Col1a1 locus, thus adapting the ES cell line for RMCE. The vector contains a tetracycline responsive promoter (TRE;tetO) controlling expression of a GFP reporter and short-hairpin RNA sequence targeting Stat3 embedded in the Mir30 structure. (J:239149)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Col1a1 Mutation:	160 strains or lines available

Original:	J:239149 Alorro MG, et al., Generation of an inducible mouse model to reversibly silence Stat3. Genesis. 2017 Apr;55(4)
All:	1 reference(s)