Gene Expression Data
Assay Details
Assay
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Reference:
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J:263979
Harley RJ, et al., Neuronal cell adhesion molecule (NrCAM) is expressed by sensory cells in the cochlea and is necessary for proper cochlear innervation and sensory domain patterning during development. Dev Dyn. 2018 Mar 14;
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6256951
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Gene symbol:
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Gap43
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Gene name:
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growth associated protein 43
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Antibody:
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anti-GAP43
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
3B:
postnatal day 2
(more )
Note: Double labeled: green - Gap43; purple - Nefh (assay MGI:6241346).
Specimen
3B:
(close )
Genetic Background:
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either: CD-1 or NIH Swiss
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Gap43; purple - Nefh (assay MGI:6241346).
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Specimen
3E:
postnatal day 2
(more )
Note: Double labeled: green - Gap43; purple - Nefh (assay MGI:6241346).
Specimen
3E:
(close )
Genetic Background:
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either: CD-1 or NIH Swiss
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Gap43; purple - Nefh (assay MGI:6241346).
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Specimen
3K:
postnatal day 2; Nrcamtm1Gmt/Nrcam +
(more )
Specimen
3K:
(close )
Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Nrcamtm1Gmt/Nrcam+
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3L:
(close )
Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Nrcamtm1Gmt/Nrcamtm1Gmt
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS27: cochlear ganglion |
Weak |
Regionally restricted |
3L
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Expression is in the efferent fibers and is further reduced around the inner hair cell region.
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TS27: organ of Corti |
Weak |
Regionally restricted |
3L
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Expression is in the efferent fibers and is further reduced around the inner hair cell region.
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Specimen
4I:
embryonic day 15.5
(more )
Note: Double labeled: green - Gap43; red - Tubb3 (assay MGI:6256952).
Specimen
4I:
(close )
Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Gap43; red - Tubb3 (assay MGI:6256952).
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Specimen
4J:
(close )
Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Nrcamtm1Gmt/Nrcamtm1Gmt
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Gap43; red - Tubb3 (assay MGI:6256952).
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Structure |
Level |
Pattern |
Image |
Note |
TS23: cochlea |
Present |
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4J
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Expression in this mutant is the same as in wild-type.
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