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Gene Expression Data
Assay Details
Assay
Reference: J:130427 Ohsugi M, et al., Maternally derived FILIA-MATER complex localizes asymmetrically in cleavage-stage mouse embryos. Development. 2008 Jan;135(2):259-69
Assay type: RT-PCR
MGI Accession ID: MGI:6256777
Gene symbol: Khdc3
Gene name: KH domain containing 3, subcortical maternal complex member
Probe: Filia-pE, Filia-pF
Assay notes: Quantitative RT-PCR. The amount of 1.2 kb Filia (Khdc3) in mid-sized oocytes (50 um) was set as 1.0. See MGI:6256765 for probe sequence.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
Oocytes (50 um) P TS28: oocyte Present (a) Not Specified; poly-A+ RNA Not Specified Female
Oocytes (75 um) P TS28: oocyte Present (b) Not Specified; poly-A+ RNA Not Specified Female
Egg P TS28: secondary oocyte Absent Not Specified; poly-A+ RNA Not Specified Female
2-Cell E1.0 (c) TS2: 2-cell stage conceptus Absent Not Specified; poly-A+ RNA Not Specified Not Specified
Morula E3.0 (c) TS3: morula-stage conceptus Present (d) Not Specified; poly-A+ RNA Not Specified Not Specified
Blastocyst E3.5 TS4: blastocyst Present (e) Not Specified; poly-A+ RNA Not Specified Not Specified
Notes:
(a) The 1.6 kb isoform was ~20% as abundant as the 1.2 kb isoform in growing oocytes (50 um).
(b) The 1.6 kb isoform appeared to diminish in fully grown oocytes (75 um).
(c) Age assigned by curator based on morphological criteria supplied by authors.
(d) The amount of the 1.6 kb isoform was greater than the 1.2 kb isoform in morula.
(e) The amount of the 1.6 kb isoform was greater than the 1.2 kb isoform in early blastocysts.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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