Probe:	Hoxd10 probe1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS20: zeugopod	Present	Regionally restricted	1E	Expression in the presumptive zeugopod domain shows a posterior bias.
TS20: limb digit	Present		1E

Structure	Level	Pattern	Image	Note
TS20: zeugopod	Present	Regionally restricted	1F	Expression in the presumptive zeugopod domain of the mutant was unchanged compared with wild type.
TS20: limb digit	Present		1F	Expression in the digit-forming region is mostly unaffected by the deletion.

Structure	Level	Pattern	Image	Note
TS20: zeugopod	Present		1K	Expression is maintained at E12.5.
TS20: limb digit	Present		1K
TS20: limb digit 1	Absent		1K

Structure	Level	Pattern	Image	Note
TS20: zeugopod	Present		1L	Expression is maintained at E12.5.
TS20: limb digit	Present		1L	Expression in the digit-forming region is mostly unaffected by the deletion. Although there was an ectopic anterior expansion into digit 1 territory.
TS20: limb digit 1	Present		1L

Structure	Level	Pattern	Image	Note
TS20: limb digit	Present	Non-Uniform	2G	Expression was in the digit region, excluding presumptive digit 1.

Structure	Level	Pattern	Image	Note
TS20: limb digit	Present	Non-Uniform	2H	Ectopic expression was detected in the presumptive digit 1 region.

Structure	Level	Pattern	Image	Note
TS20: limb digit	Present	Non-Uniform	2I	Ectopic expression was detected in the presumptive digit 1 region.

Structure	Level	Pattern	Image	Note
TS20: limb digit	Present	Non-Uniform	2P	Expression was in the digit region, excluding presumptive digit 1.
TS20: limb digit 1	Absent		2P

Structure	Level	Pattern	Image	Note
TS20: limb digit	Present	Non-Uniform	2Q	Ectopic expression was detected in the presumptive digit 1 region.
TS20: limb digit 1	Present		2Q

Structure	Level	Pattern	Image	Note
TS20: limb digit	Present	Non-Uniform	2R	Ectopic expression was detected in the presumptive digit 1 region.
TS20: limb digit 1	Present		2R

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present		3A

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present		3D	There was no alteration in the phase one expression in the early mutant limb buds.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present		5E

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present		5F	In the mutant, the separation between the phase one and phase two expression was lost.