Probe:	MDK2 cDNA2
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS24: brain	Present	Regionally restricted	4A	Only expressed in the epithelial lining of the brain and its ventricles.
TS24: peripheral nervous system ganglion	Absent		4A
TS24: spinal cord	Absent		4A
TS24: oral cavity	Present		4A
TS24: submandibular gland	Present		4A
TS24: tongue	Present		4A
TS24: trapezius	Present		4A
TS24: heart left ventricle	Present		4A
TS24: lung	Present		4A
TS24: thymus	Present		4A
TS24: heart	Present		4A	With the prominent center of the left ventricle.
TS24: blood vessel	Present	Regionally restricted	4A	Expressed in the larger ones.
TS24: liver	Ambiguous		4A	Largely unlabeled.
TS24: gut epithelium	Present	Regionally restricted	4A	Expressed in the basal epithelial layers, whereas the luminal portion of the intestinal epithelium remained largely negative.

Structure	Level	Pattern	Image	Note
TS24: brain ventricle	Present	Regionally restricted	5A	Expressed in the entire lining, not the neural tissue.
TS24: cerebral cortex	Present	Regionally restricted	5A	Expressed in the entire lining, not the neural tissue.
TS24: diencephalon	Present	Regionally restricted	5A	Expressed in the entire lining, not the neural tissue.
TS24: medulla oblongata	Present	Regionally restricted	5A	Expressed in the entire lining, not the neural tissue.
TS24: pinna	Present		5A

Structure	Level	Pattern	Image	Note
TS24: midbrain roof plate	Present	Regionally restricted	5B	Expressed in the lining of the roof of the midbrain.
TS24: midbrain vascular element	Present	Regionally restricted	5B	Expressed iin linear structures that appeared to correspond to small blood vessels protruding into the neural tissue.

Structure	Level	Pattern	Image	Note
TS24: thymus	Present	Regionally restricted	5C	Expressed in the center of the thymus as a speckled silver label, which represents interspersed epithelial or endothelial cells.
TS24: sternum	Present	Regionally restricted	5C	Expressed in the primordium of the sterno-clavicular joint.
TS24: rib	Present	Regionally restricted	5C	Expressed in the periostal collars of the dorsal rib shafts.

Structure	Level	Pattern	Image	Note
TS24: lung	Present	Widespread	5D	Expressed in the entire pulmonary tissue.
TS24: bronchus epithelium	Present	Regionally restricted	5D	Expressed in the innermost lining epithelium of a large segmental bronchus.

Structure	Level	Pattern	Image	Note
TS24: renal cortex	Present		5E
TS24: renal medulla	Present		5E
TS24: renal pelvis	Present		5E
TS24: renal vasculature	Strong		5E