Gene Expression Data
Assay Details
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Assay
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Reference:
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J:23260
Guillemot F, et al., Genomic imprinting of Mash2, a mouse gene required for trophoblast development. Nat Genet. 1995 Mar;9(3):235-42
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6231190
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Gene symbol:
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Ascl2
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Gene name:
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achaete-scute family bHLH transcription factor 2
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Probe:
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Mash2 cDNA2
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Probe preparation:
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Antisense labelled with S35 RNA
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Results
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Specimen
3a:
embryonic day 7.5
(more  )
Specimen
3a:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3b:
embryonic day 7.5; Ascl2tm1Alj/Ascl2 +
(more )
Specimen
3b:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129 * CD-1)
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Mutant Allele(s):
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Ascl2tm1Alj/Ascl2+
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: trophectoderm |
Present |
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3b
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Authors state transcripts from maternal allele have the same distribution of expression as in +/+ trophoblasts.
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Specimen
3c:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129 * CD-1)
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Mutant Allele(s):
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Ascl2tm1Alj/Ascl2tm1Alj
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3d:
embryonic day 7.5; Ascl2tm1Alj/Ascl2 +
(more )
Specimen
3d:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129 * CD-1)
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Mutant Allele(s):
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Ascl2tm1Alj/Ascl2+
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: trophectoderm |
Present |
Regionally restricted |
3d
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Authors report expression of paternal allele in groups of cells in the chorion and/or ectoplacental cone of different sizes and locations in different conceptuses. No expression in rest of trophoblast tissue.
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Specimen
3e:
embryonic day 7.5; Ascl2tm1Alj/Ascl2 +
(more )
Specimen
3e:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129 * CD-1)
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Mutant Allele(s):
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Ascl2tm1Alj/Ascl2+
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS11: trophectoderm |
Present |
Regionally restricted |
3e
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Authors report expression of paternal allele in groups of cells in the chorion and/or ectoplacental cone of different sizes and locations in different conceptuses. No expression in rest of trophoblast tissue.
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Specimen
3f:
embryonic day 7.5; Ascl2tm1Alj/Ascl2 +
(more )
Specimen
3f:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129 * CD-1)
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Mutant Allele(s):
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Ascl2tm1Alj/Ascl2+
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS11: trophectoderm |
Present |
Regionally restricted |
3f
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Authors report expression of paternal allele in groups of cells in the chorion and/or ectoplacental cone of different sizes and locations in different conceptuses. No expression in rest of trophoblast tissue.
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Specimen
4a:
embryonic day 6.5
(more )
Specimen
4a:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 6.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
4b/c:
embryonic day 6.5; Ascl2tm1Alj/Ascl2 +
(more )
Specimen
4b/c:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129 * CD-1)
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Mutant Allele(s):
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Ascl2tm1Alj/Ascl2+
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Age:
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embryonic day 6.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS9: ectoplacental cone |
Strong |
Regionally restricted |
4b/c
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Authors state that most sections did not show expression of paternal allele. However, a few sections show high level of expression in groups of ectoplacental cone cells.
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Specimen
4d/e:
embryonic day 8.5
(more )
Specimen
4d/e:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
4f:
embryonic day 8.5; Ascl2tm1Alj/Ascl2 +
(more )
Specimen
4f:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129 * CD-1)
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Mutant Allele(s):
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Ascl2tm1Alj/Ascl2+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: trophectoderm |
Present |
Regionally restricted |
4f
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Authors state that most sections did not show expression of paternal allele. However, a few sections show expression in scattered cells between chorion and giant cell layers.
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Specimen
E9.5 M-/P+ not shown:
embryonic day 9.5; Ascl2tm1Alj/Ascl2 +
(more )
Specimen
E9.5 M-/P+ not shown:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129 * CD-1)
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Mutant Allele(s):
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Ascl2tm1Alj/Ascl2+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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