Antibody:	anti-neurofilament clone 2H3
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS16: trigeminal ganglion	Present		1B	The primordia was cleary visible. Description of the pattern was summarized in Table 2.
TS16: facio-acoustic ganglion complex	Present		1B	The primordia was cleary visible. Description of the pattern was summarized in Table 2.
TS16: vagus ganglion	Present		1B	The primordia was cleary visible. Description of the pattern was summarized in Table 2.
TS16: glossopharyngeal ganglion	Present	Regionally restricted	1B	The primordium of the glossopharyngeal nerve was also present but appeared less clearly than the others. Only very few hypoglossal axons were observed.

Structure	Level	Pattern	Image	Note
TS16: facio-acoustic ganglion complex	Present		1C	The pattern of labelling does not differ greatly from the previous stage. The number of neurofilament-positive cells or fibers appears increased. Description of the pattern was summarized in Table 2.
TS16: glossopharyngeal ganglion	Present		1C	The pattern of labelling does not differ greatly from the previous stage. The number of neurofilament-positive cells or fibers appears increased. Description of the pattern was summarized in Table 2.
TS16: trigeminal ganglion	Present		1C	The pattern of labelling does not differ greatly from the previous stage. The number of neurofilament-positive cells or fibers appears increased. Description of the pattern was summarized in Table 2.
TS16: vagus ganglion	Present		1C	The pattern of labelling does not differ greatly from the previous stage. The number of neurofilament-positive cells or fibers appears increased. Description of the pattern was summarized in Table 2.

Structure	Level	Pattern	Image	Note
TS16: facio-acoustic ganglion complex	Present		1D	Description of the pattern was summarized in Table 2.
TS16: glossopharyngeal ganglion	Present		1D	Description of the pattern was summarized in Table 2.
TS16: trigeminal ganglion	Present		1D	Description of the pattern was summarized in Table 2.
TS16: vagus ganglion	Present		1D	Description of the pattern was summarized in Table 2.

Structure	Level	Pattern	Image	Note
TS17: cranial nerve	Present		1E	The fibers associated with several of the cranial nerves are more clearly labelled.
TS17: acoustic ganglion	Present		1E	Description of the pattern was summarized in Table 2.
TS17: facial ganglion	Present		1E	Description of the pattern was summarized in Table 2.
TS17: glossopharyngeal ganglion	Present		1E	Description of the pattern was summarized in Table 2.
TS17: trigeminal ganglion	Present		1E	Description of the pattern was summarized in Table 2.
TS17: vagus ganglion	Present		1E	Description of the pattern was summarized in Table 2.

Structure	Level	Pattern	Image	Note
TS17: acoustic ganglion	Present		1F	The description is similar to embryos of 31-32 somites (see Table 2).
TS17: cranial nerve	Present		1F	The description is similar to embryos of 31-32 somites (see Table 2).
TS17: facial ganglion	Present		1F	The description is similar to embryos of 31-32 somites (see Table 2).
TS17: glossopharyngeal ganglion	Present		1F	The description is similar to embryos of 31-32 somites (see Table 2).
TS17: trigeminal ganglion	Present		1F	The description is similar to embryos of 31-32 somites (see Table 2).
TS17: vagus ganglion	Present		1F	The description is similar to embryos of 31-32 somites (see Table 2).

Structure	Level	Pattern	Image	Note
TS17: acoustic ganglion	Present		2A
TS17: cranial nerve	Present		2A
TS17: facial ganglion	Present		2A
TS17: glossopharyngeal ganglion	Present		2A
TS17: trigeminal ganglion	Present		2A
TS17: vagus ganglion	Present		2A