Gene Expression Data
Assay Details
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Assay
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Reference:
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J:44409
Gofflot F, et al., Genetic patterning of the developing mouse tail at the time of posterior neuropore closure. Dev Dyn. 1997 Dec;210(4):431-45
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6226077
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Gene symbol:
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Evx1
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Gene name:
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even-skipped homeobox 1
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Probe:
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Evx1 probe1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
2E:
embryonic day 10.5
(more  )
Note: 28-30 somite stage.
Specimen
2E:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 10.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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28-30 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: tail mesenchyme |
Strong |
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2E
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At the level of the ventral curvature of the tail, a diffuse domain of expression was noted in the ventromedial mesenchyme, adjacent to the ventral ectodermal groove.
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| TS17: neural tube |
Present |
Regionally restricted |
2E
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High expression was detected at the caudal end of the neural tube, including the tissue surrounding the neuropore.
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Specimen
3a:
embryonic day 10.5
(more )
Note: Fig. 3 sections are presented in a caudal to cranial sequence (a-e).
Specimen
3a:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 10.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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Fig. 3 sections are presented in a caudal to cranial sequence (a-e).
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Specimen
3b:
embryonic day 10.5
(more )
Note: Fig. 3 sections are presented in a caudal to cranial sequence (a-e).
Specimen
3b:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 10.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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Fig. 3 sections are presented in a caudal to cranial sequence (a-e).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: future spinal cord |
Strong |
Regionally restricted |
3_Evx1 b
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Very high expression was observed in the open neuroepithelium of the caudal neuropore and in the recently closed neural tube.
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Specimen
3c:
embryonic day 10.5
(more )
Note: Fig. 3 sections are presented in a caudal to cranial sequence (a-e).
Specimen
3c:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 10.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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Fig. 3 sections are presented in a caudal to cranial sequence (a-e).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube |
Present |
Regionally restricted |
3_Evx1 c
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Intensity of staining decreased and was lost from the roof of the neural tube.
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Specimen
3d:
embryonic day 10.5
(more )
Note: Fig. 3 sections are presented in a caudal to cranial sequence (a-e).
Specimen
3d:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 10.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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Fig. 3 sections are presented in a caudal to cranial sequence (a-e).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindgut |
Present |
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3_Evx1 d
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Expression was concentrated in the apical part of the cells.
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Specimen
3e:
embryonic day 10.5
(more )
Note: Fig. 3 sections are presented in a caudal to cranial sequence (a-e).
Specimen
3e:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 10.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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Fig. 3 sections are presented in a caudal to cranial sequence (a-e).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindgut |
Present |
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3_Evx1 e
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Expression was concentrated in the apical part of the cells.
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