Reporter:	lacZ
	Direct Detection
Assay notes:	Specimens were fixed in 0.1% glutaraldehyde, 1.5% formaldehyde and were post-fixed in 4% paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS17: outflow tract cardiac muscle	Present	Regionally restricted	1A	Expression is in the myocardium underlying the cushions.
TS17: atrioventricular endocardial cushion	Present		1A
TS17: outflow tract endothelium	Present		1A
TS17: outflow tract endocardial cushion	Present		1A	Expression is in the mesenchymal cells.

Structure	Level	Pattern	Image	Note
TS20: heart valve primordium	Strong	Regionally restricted	1B	Expression is strong in the annulus and weak in the valvular mesenchyme.
TS20: heart	Strong	Regionally restricted	1B	Expression is in the tendinous cords and connective tissue supporting the semilunar valves.
TS20: atrioventricular canal	Weak		1B
TS20: outflow tract endocardial cushion	Weak		1B

Structure	Level	Pattern	Image	Note
TS20: heart valve primordium	Strong	Regionally restricted	1C	Expression is strong in the annulus and endothelium and is weak in the valvular mesenchyme.
TS20: heart	Strong	Regionally restricted	1C	Expression is in the tendinous cords and connective tissue supporting the semilunar valves.
TS20: atrioventricular canal	Weak		1C
TS20: outflow tract endocardial cushion	Weak		1C

Structure	Level	Pattern	Image	Note
TS22: heart valve	Strong	Regionally restricted	1D	Expression is strong in the annulus and endothelium and is weak in the valvular mesenchyme.
TS22: heart	Strong	Regionally restricted	1D	Expression is in the tendinous cords and connective tissue supporting the semilunar valves.
TS22: atrioventricular canal	Weak		1D
TS22: outflow tract	Weak		1D

Structure	Level	Pattern	Image	Note
TS22: heart valve	Strong	Regionally restricted	1E , 1E'	Expression is strong in the annulus and endothelium at the tips and is weak in the valvular mesenchyme.
TS22: heart	Strong	Regionally restricted	1E , 1E'	Expression is in the tendinous cords and connective tissue supporting the semilunar valves.
TS22: atrioventricular canal	Weak	Regionally restricted	1E' , 1E
TS22: outflow tract	Weak	Regionally restricted	1E' , 1E

Structure	Level	Pattern	Image	Note
TS26: heart valve	Strong	Regionally restricted	1F' , 1F	Expression is strong in the annulus and endothelium at the tips and is weak in the valvular mesenchyme.
TS26: chordae tendinae	Strong		1F' , 1F
TS26: heart	Strong	Regionally restricted	1F' , 1F	Expression is in the connective tissue supporting the semilunar valves.
TS26: atrioventricular endocardial cushion	Weak		1F , 1F'
TS26: outflow tract	Weak		1F , 1F'
TS26: semilunar valve	Weak		1F , 1F'

Structure	Level	Pattern	Image	Note
TS26: heart valve	Strong	Regionally restricted	1G , 1G'	Expression is strong in the annulus and endothelium at the tips and is weak in the valvular mesenchyme.
TS26: heart	Strong	Regionally restricted	1G' , 1G	Expression is in the connective tissue supporting the semilunar valves.
TS26: atrioventricular endocardial cushion	Weak		1G' , 1G
TS26: outflow tract	Weak		1G' , 1G
TS26: semilunar valve	Weak		1G' , 1G