Gene Expression Data
Assay Details
Assay
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Reference:
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J:156768
Mizuhara E, et al., Purkinje cells originate from cerebellar ventricular zone progenitors positive for Neph3 and E-cadherin. Dev Biol. 2010 Feb 15;338(2):202-14
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6220661
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Gene symbol:
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Kirrel2
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Gene name:
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kirre like nephrin family adhesion molecule 2
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Results
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Specimen
1Ab:
embryonic day 12.5
(more )
Note: Double labeled: red - Neph3 (Kirrel2); green - Lbx1 (assay MGI:6220662).
Specimen
1Ab:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Neph3 (Kirrel2); green - Lbx1 (assay MGI:6220662).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord ventricular layer |
Present |
Regionally restricted |
1Ab
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Expression restricted to dorsal part of ventricular zone. Selective expression in subpopulation of ventricular zone cells in spinal dIL domain.
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Specimen
4Aa:
embryonic day 12.5
(more )
Note: Double labeled: red - Neph3 (Kirrel2); green - Lhx1 (assay MGI:6220664).
Specimen
4Aa:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Neph3 (Kirrel2); green - Lhx1 (assay MGI:6220664).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: cerebellum primordium |
Present |
Regionally restricted |
4Aa
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Authors report selective expression in ventricular zone cells neighboring the mantle layer containing GABAergic neurons.
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Specimen
4Ab:
embryonic day 12.5
(more )
Note: Double labeled: red - Neph3 (Kirrel2); green - Corl2 (Skor2 assay MGI:6220665).
Specimen
4Ab:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Embedding:
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Cryosection
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Note:
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Double labeled: red - Neph3 (Kirrel2); green - Corl2 (Skor2 assay MGI:6220665).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: cerebellum primordium |
Present |
Regionally restricted |
4Ab
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Authors report selective expression in ventricular zone cells neighboring the mantle layer containing GABAergic neurons.
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Specimen
4Ac:
embryonic day 12.5
(more )
Note: Double labeled: red - Neph3 (Kirrel2); green - Pax2 (assay MGI:6220666).
Specimen
4Ac:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Note:
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Double labeled: red - Neph3 (Kirrel2); green - Pax2 (assay MGI:6220666).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: cerebellum primordium |
Present |
Regionally restricted |
4Ac
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Authors report selective expression in ventricular zone cells neighboring the mantle layer containing GABAergic neurons.
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Specimen
7Ca,a":
embryonic day 12.5
(more )
Note: Triple labeled: red - Neph3 (Kirrel2); green - E-cad (Cdh1 assay MGI:6220667); blue - Corl2 (Skor2 assay MGI:6220665).
Specimen
7Ca,a":
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: red - Neph3 (Kirrel2); green - E-cad (Cdh1 assay MGI:6220667); blue - Corl2 (Skor2 assay MGI:6220665).
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Specimen
7Cb,b":
embryonic day 12.5
(more )
Note: Triple labeled: red - Neph3 (Kirrel2); green - E-cad (Cdh1 assay MGI:6220667); blue - Pax2 (assay MGI:6220666).
Specimen
7Cb,b":
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: red - Neph3 (Kirrel2); green - E-cad (Cdh1 assay MGI:6220667); blue - Pax2 (assay MGI:6220666).
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Specimen
7Cc:
embryonic day 12.5
(more )
Note: Triple labeled: red - Neph3 (Kirrel2); green - E-cad (Cdh1 assay MGI:6220667); blue - Pax2 (assay MGI:6220666).
Specimen
7Cc:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: red - Neph3 (Kirrel2); green - E-cad (Cdh1 assay MGI:6220667); blue - Pax2 (assay MGI:6220666).
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Specimen
7Cd:
embryonic day 12.5
(more )
Note: Triple labeled: red - Neph3 (Kirrel2); green - E-cad (Cdh1 assay MGI:6220667); blue - Pax2 (assay MGI:6220666).
Specimen
7Cd:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: red - Neph3 (Kirrel2); green - E-cad (Cdh1 assay MGI:6220667); blue - Pax2 (assay MGI:6220666).
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Specimen
7Ce:
embryonic day 14.5
(more )
Note: Double labeled: red - Neph3 (Kirrel2); green - E-cad (Cdh1 assay MGI:6220667).
Specimen
7Ce:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Neph3 (Kirrel2); green - E-cad (Cdh1 assay MGI:6220667).
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Specimen
S2:
embryonic day 10.5
(more )
Note: Triple labeled: red - Neph3 (Kirrel2); red - Pax2 (assay MGI:6220666); blue - Mash1 (Ascl1 assay MGI:6220668);
Specimen
S2:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: red - Neph3 (Kirrel2); red - Pax2 (assay MGI:6220666); blue - Mash1 (Ascl1 assay MGI:6220668);
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Structure |
Level |
Pattern |
Image |
Note |
TS17: future spinal cord |
Present |
Regionally restricted |
S2
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Expression restricted to dI4 precursor domain.
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