Gene Expression Data
Assay Details
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Assay
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Reference:
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J:153643
Self M, et al., Six2 activity is required for the formation of the mammalian pyloric sphincter. Dev Biol. 2009 Oct 15;334(2):409-17
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6214819
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Gene symbol:
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Acta2
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Gene name:
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actin alpha 2, smooth muscle, aorta
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Antibody:
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anti-alpha-SMA
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Detection system:
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Secondary antibody coupled to Cy3
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Results
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Specimen
3A:
embryonic day 14.5
(more  )
Specimen
3A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: pyloric sphincter |
Present |
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3A
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Expression in stomach constriction of gut tube at level of developing pyloric sphincter.
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Specimen
3B:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Six2tm1Gco/Six2tm1Gco
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: pyloric sphincter |
Present |
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3B
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Authors state that constriction of the gut tube at the boundary of stomach and small intestine does not form.
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Specimen
3C:
embryonic day 14.5
(more )
Specimen
3C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: stomach mesenchyme |
Present |
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3C
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Expression in stomach reveals thickening of smooth muscle layer.
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Specimen
3D:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Six2tm1Gco/Six2tm1Gco
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3E:
embryonic day 18.5
(more )
Specimen
3E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: pyloric sphincter |
Present |
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3E
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Characteristic thickening of smooth muscle layer and constriction of the gut tube in pyloric sphincter region.
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Specimen
3F:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Six2tm1Gco/Six2tm1Gco
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: foregut |
Present |
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3F
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No evidence of pyloric sphincter formation at the stomach-small intestine junction.
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Specimen
3G:
embryonic day 18.5
(more )
Specimen
3G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3H:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Six2tm1Gco/Six2tm1Gco
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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