Gene Expression Data
Assay Details
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Assay
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Reference:
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J:149254
Caton J, et al., Enamel-free teeth: Tbx1 deletion affects amelogenesis in rodent incisors. Dev Biol. 2009 Apr 15;328(2):493-505
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6209567
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Gene symbol:
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Tbx1
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Gene name:
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T-box 1
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Probe:
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Tbx1 probe9
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
1A:
embryonic day 10.0
(more  )
Specimen
1A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: oral epithelium |
Weak |
Regionally restricted |
1A
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Weak expression in both presumptive incisor and molar areas.
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Specimen
1B:
embryonic day 10.5-11.0
(more )
Specimen
1B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5-11.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: oral epithelium |
Strong |
Regionally restricted |
1B
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Stronger expression, expecially in the most distal areas where incisors develop.
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Specimen
1C:
embryonic day 10.5-11.0
(more )
Specimen
1C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5-11.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: oral epithelium |
Strong |
Regionally restricted |
1C
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Stronger expression, expecially in the most distal areas where incisors develop.
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Specimen
1D:
embryonic day 13.5
(more )
Note: Bud stage.
Specimen
1D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Bud stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: upper jaw incisor epithelium |
Present |
Regionally restricted |
1D
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Expression in epithelial cells in proximity to dental papilla mesenchyme. No expression in central part and flanks of maxillary and mandibular incisor germs.
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Specimen
1E:
embryonic day 13.5
(more )
Note: Bud stage.
Specimen
1E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Bud stage.
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Specimen
1F:
embryonic day 14.0
(more )
Note: Cap stage.
Specimen
1F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Cap stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: upper jaw incisor epithelium |
Present |
Regionally restricted |
1F
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Authors report restricted expression pattern maintained in epithelium during rotation of maxillary and mandibular incisors.
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Specimen
1G:
embryonic day 14.0
(more )
Note: Cap stage.
Specimen
1G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Cap stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: lower jaw incisor epithelium |
Present |
Regionally restricted |
1G
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Authors report restricted expression pattern maintained in epithelium during rotation of maxillary and mandibular incisors.
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Specimen
1H:
embryonic day 14.5
(more )
Note: Cap stage.
Specimen
1H:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Cap stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: upper jaw incisor epithelium |
Present |
Regionally restricted |
1H
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Authors report restricted expression pattern maintained in epithelium during rotation of maxillary and mandibular incisors.
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Specimen
1I:
embryonic day 14.5
(more )
Note: Cap stage.
Specimen
1I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Cap stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: lower jaw incisor epithelium |
Present |
Regionally restricted |
1I
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Authors report restricted expression pattern maintained in epithelium during rotation of maxillary and mandibular incisors.
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Specimen
1J:
embryonic day 18.5
(more )
Note: Bell stage.
Specimen
1J:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Bell stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: incisor epithelium |
Present |
Regionally restricted |
1J
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Expression at posterior end of incisor in inner dental epithelium at labial side. Expression in cells of outer dental epithelium at very posterior end of incisor.
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Specimen
1J left box:
embryonic day 18.5
(more )
Note: Bell stage.
Specimen
1J left box:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Bell stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: incisor epithelium |
Present |
Regionally restricted |
1J left box
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No expression in rest of dental epithelium extending anteriorly (i.e. preameloblasts, ameloblasts, outer dental epithelium) on labial side.
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Specimen
1J right box:
embryonic day 18.5
(more )
Note: Bell stage.
Specimen
1J right box:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Bell stage.
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Specimen
10A:
embryonic day 16.5
(more )
Specimen
10A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
10B:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Spry2tm1.1Mrt/Spry2+
Spry4tm1.2Mrt/Spry4tm1.2Mrt
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: incisor epithelium |
Present |
Regionally restricted |
10B
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Expression on both lingual and labial sides. Ectopic expression on lingual side restricted to posterior end of tooth.
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