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Gene Expression Data
Assay Details
Assay
Reference: J:173606 Oqani RK, et al., The CDK9/Cyclin T1 subunits of P-TEFb in mouse oocytes and preimplantation embryos: A possible role in embryonic genome activation. BMC Dev Biol. 2011;11:33
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6203136
Gene symbol: Ccnt1
Gene name: cyclin T1
Antibody: anti-cyclin T1 (sc-271575)
Detection system: Secondary antibody coupled to fluorescein
Assay notes: Fixative was 2% formaldehyde. Relative intensities of fluorescent signals from the double labeled specimens are shown in figure 1D.
Results
Specimen 1A: embryonic day 1.0 (more )
Note: Late 2-cell stage embryo.
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present 1A Cyclin T1
Specimen 1B GV: postnatal adult (more )
Note: Germinal vesicle oocyte. Double labeled: red - Ccnt1; green - Cdk9 (assay MGI:6203134).
Structure Level Pattern Image Note
TS28: primary oocyte Weak 1B GV Merge , 1B GV Cyclin T1
Specimen 1B MII: postnatal adult (more )
Note: Metaphase II oocyte. Double labeled: red - Ccnt1; green - Cdk9 (assay MGI:6203134).
Structure Level Pattern Image Note
TS28: secondary oocyte Weak 1B MII Cyclin T1 , 1B MII Merge Expression is cytoplasmic.
Specimen 1C 1-cell: embryonic day 0.5 (more )
Note: Double labeled: red - Ccnt1; green - Cdk9 (assay MGI:6203134).
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present 1C 1-cell Cyclin T1 , 1C 1-cell Merge Expression is cytoplasmic.
Specimen 1C mid 2-cell: embryonic day 1.0 (more )
Note: Double labeled: red - Ccnt1; green - Cdk9 (assay MGI:6203134).
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present 1C mid 2-cell Merge , 1C mid 2-cell Cyclin T1
Specimen 1C late 2-cell: embryonic day 1.5 (more )
Note: Double labeled: red - Ccnt1; green - Cdk9 (assay MGI:6203134).
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present 1C late 2-cell Cyclin T1 , 1C late 2-cell Merge Expression is predominantly nuclear.
Specimen 1C 4-cell: embryonic day 2.0 (more )
Note: Double labeled: red - Ccnt1; green - Cdk9 (assay MGI:6203134).
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Present 1C 4-cell Merge , 1C 4-cell Cyclin T1 Expression is nuclear.
Specimen 1C 8-cell: embryonic day 2.5 (more )
Note: Double labeled: red - Ccnt1; green - Cdk9 (assay MGI:6203134).
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present 1C 8-cell Cyclin T1 , 1C 8-cell Merge Expression is nuclear.
Specimen 1C morula: embryonic day 3.0 (more )
Note: Double labeled: red - Ccnt1; green - Cdk9 (assay MGI:6203134).
Structure Level Pattern Image Note
TS4: compacted morula Present 1C morula Merge , 1C morula Cyclin T1 Expression is nuclear.
Specimen 1C blastocyst: embryonic day 3.5 (more )
Note: Double labeled: red - Ccnt1; green - Cdk9 (assay MGI:6203134).
Structure Level Pattern Image Note
TS4: inner cell mass Present 1C Blastocyst Merge , 1C Blastocyst Cyclin T1 Expression is nuclear.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory