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Gene Expression Data
Assay Details
Assay
Reference: J:196288 Nechiporuk T, et al., Dlg5 maintains apical aPKC and regulates progenitor differentiation during lung morphogenesis. Dev Biol. 2013 May 15;377(2):375-84
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6203100
Gene symbol: Prkci
Gene name: protein kinase C, iota
Antibody: Anti-aPKC
Detection system: Secondary antibody coupled to Texas Red
Assay notes: The specificity of the antibody used was not detailed; both family members have been annotated.
Results
Specimen 5B/C: embryonic day 14.5 (more )
Note: Double labeled: red - Prkci and Prkcz; green - Dlg5 (assay MGI:6203057).
Structure Level Pattern Image Note
TS22: lung epithelium Present 5C , 5D , 5B Expression was localized to the apical membrane domains.
Specimen 5B'/C': embryonic day 14.5; Dlg5tm1Vv/Dlg5tm1Vv (more )
Note: Double labeled: red - Prkci and Prkcz; green - Dlg5 (assay MGI:6203057).
Structure Level Pattern Image Note
TS22: lung epithelium Present 5C' , 5B' , 5D' Apical staining was lost and there was prominent lateral membrane staining.
Specimen 5F/G: embryonic day 14.5 (more )
Note: Double labeled: red - Prkci and Prkcz; green - Cdh1 (assay MGI:6203107).
Structure Level Pattern Image Note
TS22: lung epithelium Present 5F , 5H , 5G Expression was localized to the apical membrane domains.
Specimen 5F'/G': embryonic day 14.5; Dlg5tm1Vv/Dlg5tm1Vv (more )
Note: Double labeled: red - Prkci and Prkcz; green - Cdh1 (assay MGI:6203107).
Structure Level Pattern Image Note
TS22: lung epithelium Present 5H' , 5G' , 5F' Apical staining was lost and there was prominent lateral membrane staining.
Specimen S5B/C: embryonic day 14.5 (more )
Note: Double labeled: red - Prkci and Prkcz; green - Pard3 (assay MGI:6203115).
Structure Level Pattern Image Note
TS22: lung epithelium Present S5B , S5C Expression was localized to the apical membrane domains.
Specimen S5B'/C': embryonic day 14.5; Dlg5tm1Vv/Dlg5tm1Vv (more )
Note: Double labeled: red - Prkci and Prkcz; green - Pard3 (assay MGI:6203115).
Structure Level Pattern Image Note
TS22: lung epithelium Present S5C' , S5B' Apical staining was lost and there was prominent lateral membrane staining.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
MGI 6.23 		The Jackson Laboratory