Reporter:	lacZ
	Direct Detection

Structure	Level	Pattern	Image	Note
TS17: olfactory epithelium	Weak		1A	Expressed in apical epithelial cells in the olfactory placodes.

Structure	Level	Pattern	Image	Note
TS19: olfactory epithelium	Present		1B	More reactivity in apical and basal regions. There was differential staining intensity in different parts of the epithelium, but rather than a general gradient of staining.

Structure	Level	Pattern	Image	Note
TS20: nasal cavity epithelium	Moderate		1C	Expressed in epithelial cells in the primitive nasal cavity.
TS20: nasal mesenchyme	Moderate	Regionally restricted	1C	Expressed in mesenchy mesenchymal cells surrounding the epithelial cells.

Structure	Level	Pattern	Image	Note
TS22: olfactory epithelium	Present		1D	Fainter expression compared with that of E15.5.

Structure	Level	Pattern	Image	Note
TS23: olfactory epithelium	Strong	Regionally restricted	1E	Expressed in several regions, particularly the apical layer, although reactive cells were present throughout the height of the epithelium.
TS23: nasal mesenchyme	Present	Diffuse	1E	Paler staining beneath the epithelium itself, possibly in the axons of the olfactory receptor cells as well as in underlying mesenchyme.

Structure	Level	Pattern	Image	Note
TS24: olfactory epithelium	Strong	Regionally restricted	1F	Expressed in several regions, particularly the apical layer, although reactive cells were present throughout the height of the epithelium.
TS24: nasal mesenchyme	Present	Diffuse	1F	Paler staining beneath the epithelium itself, possibly in the axons of the olfactory receptor cells as well as in underlying mesenchyme.

Structure	Level	Pattern	Image	Note
TS25: olfactory epithelium	Strong	Regionally restricted	1G	Expressed in several regions, particularly the apical layer, although reactive cells were present throughout the height of the epithelium.
TS25: nasal mesenchyme	Present	Diffuse	1G	Paler staining beneath the epithelium itself, possibly in the axons of the olfactory receptor cells as well as in underlying mesenchyme.

Structure	Level	Pattern	Image	Note
TS25: olfactory epithelium	Strong	Regionally restricted	1H	Expressed in several regions, particularly the apical layer, although reactive cells were present throughout the height of the epithelium.
TS25: nasal mesenchyme	Present	Diffuse	1H	Paler staining beneath the epithelium itself, possibly in the axons of the olfactory receptor cells as well as in underlying mesenchyme.
TS25: olfactory nerve	Present		1H	Heavier staining in olfactory receptor neurons axons as they enter the olfactory bulb. Reactive fascicles of olfactory nerve fibers bridge between the olfactory epithelium and olfactory bulb.

Structure	Level	Pattern	Image	Note
TS24: main olfactory bulb prospective glomerular layer	Present		2A

Structure	Level	Pattern	Image	Note
TS25: main olfactory bulb prospective glomerular layer	Present		2B	Increased expression in the neuropil of protoglomeruli.

Structure	Level	Pattern	Image	Note
TS26: main olfactory bulb glomerular layer	Weak	Regionally restricted	2C	Expressed in fibers within some glomeruli and around protoglomeruli.
TS26: olfactory nerve	Weak	Regionally restricted	2C	Expressed in fibers within some glomeruli and around protoglomeruli.

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb glomerular layer	Present		2D	Increased expression in fibers within some glomeruli and around protoglomeruli.
TS27: olfactory nerve	Present		2D	Increased expression in fibers within some glomeruli and around protoglomeruli.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb olfactory nerve layer	Strong		2E	Not apparent throughout the whole layer. Some glomeruli exhibit more reactivity than others.
TS28: main olfactory bulb layer	Present	Widespread	2E	Expressed in many glomeruli (50%) widely dispersed throughout the extent of the olfactory bulb, although at mid-rostral/caudal levels nearly all glomeruli are stained.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb olfactory nerve layer	Strong		2F

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb layer	Strong		2G	Differentially reactive glomeruli, with some glomeruli heavily stained and others showing no stain at all.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb layer	Strong		2H	Differentially reactive glomeruli, with some glomeruli heavily stained and others showing no stain at all.

Structure	Level	Pattern	Image	Note
TS28: olfactory epithelium	Strong		3A	Most intense stain was in the posterior portions of endoturbinates IIa and IIb. A strong patch staining in the central region of turbinate IIb.

Structure	Level	Pattern	Image	Note
TS28: olfactory epithelium	Strong		3B	Most intense stain was in the posterior portions of endoturbinates IIa and IIb. A strong patch staining in the central region of turbinate IIb.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Strong	Regionally restricted	5A	Expressed as a band of glomeruli run from the midlateral anterior part of the ventral olfactory bulb to a more medial posterior location. Within this band of stained glomeruli lay a ventral group of large, heavily stained glomeruli (8 to 20 on each side).

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Strong	Regionally restricted	5B	Expressed as a band of glomeruli run from the midlateral anterior part of the ventral olfactory bulb to a more medial posterior location. Within this band of stained glomeruli lay a ventral group of large, heavily stained glomeruli (8 to 20 on each side).

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Strong	Regionally restricted	5C	Expressed as a band of glomeruli run from the midlateral anterior part of the ventral olfactory bulb to a more medial posterior location. Within this band of stained glomeruli lay a ventral group of large, heavily stained glomeruli (8 to 20 on each side).

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Strong	Regionally restricted	5D	Expressed as a band of glomeruli run from the midlateral anterior part of the ventral olfactory bulb to a more medial posterior location. Within this band of stained glomeruli lay a ventral group of large, heavily stained glomeruli (8 to 20 on each side).

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Strong	Regionally restricted	5E	Expressed as a band of glomeruli run from the midlateral anterior part of the ventral olfactory bulb to a more medial posterior location. Within this band of stained glomeruli lay a ventral group of large, heavily stained glomeruli (8 to 20 on each side).

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Strong	Regionally restricted	5G	Expressed as a band of glomeruli run from the midlateral anterior part of the ventral olfactory bulb to a more medial posterior location. Within this band of stained glomeruli lay a ventral group of large, heavily stained glomeruli (8 to 20 on each side).

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Strong	Regionally restricted	5H	Expressed as a band of glomeruli run from the midlateral anterior part of the ventral olfactory bulb to a more medial posterior location. Within this band of stained glomeruli lay a ventral group of large, heavily stained glomeruli (8 to 20 on each side).

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Strong	Regionally restricted	5I	Expressed as a band of glomeruli run from the midlateral anterior part of the ventral olfactory bulb to a more medial posterior location. Within this band of stained glomeruli lay a ventral group of large, heavily stained glomeruli (8 to 20 on each side).

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Strong	Regionally restricted	5J	Expressed as a band of glomeruli run from the midlateral anterior part of the ventral olfactory bulb to a more medial posterior location. Within this band of stained glomeruli lay a ventral group of large, heavily stained glomeruli (8 to 20 on each side).

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Present	Regionally restricted	8	Positive staining didn't colocalize with trigeminally innervated glomeruli. High expression in glomeruli 1 and 2 but little Calca-like immunoreactivity was present, whereas Calca-immunoreactive fibers entered glomeruli 3 and 4.

Structure	Level	Pattern	Image	Note
TS22: main olfactory bulb	Absent
TS22: olfactory nerve	Present			Expressed in olfactory nerve fibers as they travel to the olfactory bulb.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Present	Regionally restricted		Positive staining didn't colocalize with trigeminally innervated glomeruli.