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Gene Expression Data
Assay Details
Assay
Reference: J:162627 Kinzel D, et al., Pitchfork regulates primary cilia disassembly and left-right asymmetry. Dev Cell. 2010 Jul 20;19(1):66-77
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6202410
Gene symbol: Cimap3
Gene name: ciliary microtubule associated protein 3
Antibody: Anti-Pifo
Results
Specimen 1G: postnatal adult (more )
Note: Double labeled: red - Cimap3; green - acetylated tubulin. Nuclei labeled with Dapi.
Structure Level Pattern Image Note
TS28: spermatozoon Present Regionally restricted 1G Expression was localized in parcels along the sperm tail.
Specimen 1H: postnatal adult (more )
Note: Double labeled: red - Cimap3; green - acetylated tubulin. Nuclei labeled with Dapi.
Structure Level Pattern Image Note
TS28: spermatozoon Present Regionally restricted 1H Expression was localized in parcels along the sperm tail.
Specimen 1I: embryonic day 7.75 (more )
Note: Double labeled: red - Cimap3; green - acetylated tubulin. Nuclei labeled with Dapi.
Structure Level Pattern Image Note
TS11: node Present 1I , 1J Expression was detected along the stabilized microtubules of node pit cells.
Specimen S1G: embryonic day 12.5 (more )
Note: Double labeled: red - Cimap3; green - acetylated tubulin. Nuclei labeled with Dapi.
Structure Level Pattern Image Note
TS20: lung mesenchyme Present S1G
Specimen S1I: postnatal adult (more )
Note: Double labeled: red - Cimap3; green - acetylated tubulin. Nuclei labeled with Dapi.
Structure Level Pattern Image Note
TS28: testis Present S1I
Specimen S4B: embryonic day 10.5 (more )
Note: Double labeled: red - Cimap3; green - Shh (assay MGI:6202458). Nuclei labeled with Dapi.
Structure Level Pattern Image Note
TS17: neural tube floor plate Present S4B Pifo expression was detected in regions of high Shh activity.
Specimen S4D: embryonic day 10.5 (more )
Note: Double labeled: red - Cimap3; green - Shh (assay MGI:6202458). Nuclei labeled with Dapi.
Structure Level Pattern Image Note
TS17: apical ectodermal ridge Present S4D Pifo expression was detected in regions of high Shh activity.
Specimen 4B: embryonic day 7.5-7.75 (more )
Note: Double labeled: green - Cimap3; red - Cep164 (assay MGI:6202513).
Structure Level Pattern Image Note
TS11: node Present 4B Pifo colocalized with the appendage marker Cep164 in cilia-assembling and cilia-disassembling cells, but was absent from the basal body of primary ciliated node cells.
Specimen 4C: embryonic day 7.5-7.75 (more )
Note: Double labeled: green - Cimap3; red - Cep135 (assay MGI:6202516).
Structure Level Pattern Image Note
TS11: node Present 4C Pifo colocalized with the centriolar marker Cep135 in cilia-assembling and cilia-disassembling cells, but was absent from the basal body of primary ciliated node cells.
Specimen 4D cilia assembly left: embryonic day 7.5-7.75 (more )
Note: Double labeled: green - Cimap3; red - Cep135 (assay MGI:6202516).
Structure Level Pattern Image Note
TS11: node Present 4D cilia assembly Merge left , 4D cilia assembly Pifo left Pifo colocalized with the centriolar marker Cep135 in cilia-assembling cells.
Specimen 4D cilia assembly right: embryonic day 7.5-7.75 (more )
Note: Double labeled: green - Cimap3; red - Cep164 (assay MGI:6202513).
Structure Level Pattern Image Note
TS11: node Present 4D cilia assembly Merge right , 4D cilia assembly Pifo right Pifo colocalized with the centriolar marker Cep164 in cilia-assembling cells.
Specimen 4D cilia disassembly left: embryonic day 7.5-7.75 (more )
Note: Double labeled: green - Cimap3; red - Cep135 (assay MGI:6202516).
Structure Level Pattern Image Note
TS11: node Present 4D cilia disassembly Pifo left , 4D cilia disassembly Merge left Pifo colocalized with the centriolar marker Cep135 in cilia-disassembling cells.
Specimen 4D cilia disassembly right: embryonic day 7.5-7.75 (more )
Note: Double labeled: green - Cimap3; red - Cep135 (assay MGI:6202516).
Structure Level Pattern Image Note
TS11: node Present 4D cilia disassembly Pifo right , 4D cilia disassembly Merge right Pifo colocalized with the centriolar marker Cep164 in cilia-disassembling cells.
Specimen 4E ventral node pit cells 1: embryonic day 7.5-7.75 (more )
Note: Double labeled: green - Cimap3; red - Cep135 (assay MGI:6202516).
Structure Level Pattern Image Note
TS11: node Present Regionally restricted 4E Merge 1 , 4E Pifo 1 In ventral node pit cells, Pifo is localized apical to the adherens junction at the base of the primary cilium in a ciliary necklace-like pattern and colocalizes with Cep135.
Specimen 4E ventral node pit cells 2: embryonic day 7.5-7.75 (more )
Note: Double labeled: red - Cimap3; green - Kif3a (assay MGI:6202517).
Structure Level Pattern Image Note
TS11: node Present Regionally restricted 4E Merge 2 , 4E Pifo 2 In ventral node pit cells, Pifo is localized apical to the adherens junction at the base of the primary cilium in a ciliary necklace-like pattern and colocalizes with Kif3a.
Specimen 4E ventral node pit cells 3: embryonic day 7.5-7.75 (more )
Note: Double labeled: red - Cimap3; green - Cdh1 (assay MGI:6202518).
Structure Level Pattern Image Note
TS11: node Present Regionally restricted 4E Pifo 3 , 4E Merge 3 In ventral node pit cells, Pifo is localized apically to the adherens junction marker Cdh1 and at the base of the primary cilium in a ciliary necklace-like pattern.
Specimen 4E ventral node pit cells 4: embryonic day 7.5-7.75 (more )
Note: Double labeled: red - Cimap3; green - acetylated tubulin.
Structure Level Pattern Image Note
TS11: node Present Regionally restricted 4E Pifo 4 , 4E Merge 4 In ventral node pit cells, Pifo is localized apical to the adherens junction at the base of the primary cilium in a ciliary necklace-like pattern.
Specimen 4E ventral node pit cells 5: embryonic day 7.5-7.75 (more )
Note: Double labeled: red - Cimap3; green - Aurka (assay MGI:6202520).
Structure Level Pattern Image Note
TS11: node Present Regionally restricted 4E Pifo 5 , 4E Merge 5 In ventral node pit cells, Pifo is localized apical to the adherens junction at the base of the primary cilium in a ciliary necklace-like pattern and colocalizes with phosphorylated Aurka.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory