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Gene Expression Data
Assay Details
Assay
Reference: J:264681 Koehler S, et al., Construction of a viral T2A-peptide based knock-in mouse model for enhanced Cre recombinase activity and fluorescent labeling of podocytes. Kidney Int. 2017 Jun;91(6):1510-1517
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6202121
Gene symbol: Nphs2
Gene name: nephrosis 2, podocin
Antibody: anti-podocin (P0372)
Detection system: Secondary antibody
Results
Specimen 3b - 12 weeks: postnatal week 12; Nphs2tm1.1(icre)Ptb/Nphs2+ (more )
Note: Double labeled: green - Nphs2 protein; purple - Nphs2 reporter protein (assay MGI:6202340).
Structure Level Pattern Image Note
TS28: Bowman's capsule of mature renal corpuscle Present Regionally restricted 3d left , 3b left Expression is seen in the podocytes. There are no signs of glomerular disease.
Specimen 3b - 6 months: postnatal month 6; Nphs2tm1.1(icre)Ptb/Nphs2+ (more )
Note: Double labeled: green - Nphs2 protein; purple - Nphs2 reporter protein (assay MGI:6202340).
Structure Level Pattern Image Note
TS28: Bowman's capsule of mature renal corpuscle Present Regionally restricted 3b center , 3d center Expression is seen in the podocytes. There are no signs of glomerular disease.
Specimen 3b - 1 year: postnatal year 1; Nphs2tm1.1(icre)Ptb/Nphs2+ (more )
Note: Double labeled: green - Nphs2 protein; purple - Nphs2 reporter protein (assay MGI:6202340).
Structure Level Pattern Image Note
TS28: Bowman's capsule of mature renal corpuscle Present Regionally restricted 3b right , 3d right Expression is seen in the podocytes. There are no signs of glomerular disease.
Specimen 4d - upper: postnatal day 19; Phb2tm1Tlan/Phb2+, Nphs2tm1.1(icre)Ptb/Nphs2+ (more )
Note: Conditional mutant. Double labeled: green - Nphs2 protein; purple - Nphs2 reporter protein (assay MGI:6202340).
Structure Level Pattern Image Note
TS28: Bowman's capsule of mature renal corpuscle Present 4d upper right , 4d upper left
Specimen 4d - lower: postnatal day 19; Phb2tm1Tlan/Phb2tm1Tlan, Nphs2tm1.1(icre)Ptb/Nphs2+ (more )
Note: Conditional mutant. Double labeled: green - Nphs2 protein; purple - Nphs2 reporter protein (assay MGI:6202340).
Structure Level Pattern Image Note
TS28: Bowman's capsule of mature renal corpuscle Present 4d lower right , 4d lower left Expression is disrupted relative to that seen in controls.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
MGI 6.23 		The Jackson Laboratory